Clinical and Pathologic Features of Neuronal Ceroid-Lipofuscinosis in a Ferret (Mustela putorius furo)
| Autor: | Hiroyuki Nakayama, Kazuyuki Uchida, K. Uetsuka, James K. Chambers, Yasutsugu Miwa, Kazumi Nibe, Satoru Matsunaga |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Saposins Luxol fast blue stain chemistry.chemical_compound Euthanasia Animal Neuronal Ceroid-Lipofuscinoses medicine Animals Macrophage Cerebral Cortex Neurons Cerebral atrophy General Veterinary Macrophages Ferrets Brain Mitochondrial Proton-Translocating ATPases medicine.disease Immunohistochemistry Astrogliosis Autofluorescence medicine.anatomical_structure nervous system chemistry Cerebral cortex Female Neuronal ceroid lipofuscinosis Rabbits Sudan Black B Biomarkers |
| Zdroj: | Veterinary Pathology. 48:1185-1189 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/0300985811400441 |
| Popis: | Clinical and pathologic features of neuronal ceroid-lipofuscinosis in a 4-month-old ferret are reported. Clinical signs including neurological symptoms appeared at 3 months of age and progressed rapidly. By magnetic resonance imaging, severe cerebral atrophy was recognized. Histopathologically, there was severe neuronal loss and diffuse astrogliosis with macrophage accumulations; lesions were found predominantly in the cerebral cortex. Intracytoplasmic pigments were observed in surviving neurons and macrophages throughout the brain. The pigments were intensely positive for periodic acid–Schiff, Luxol fast blue, and Sudan black B and exhibited a green autofluorescence. Electron microscopic examination revealed the accumulation of electron-dense granular material within lysosomes of neurons and macrophages. Immunohistochemically, a large number of saposin-positive granules accumulated in the neuronal cells, astrocytes, and macrophages of the lesions, but significant immunoreactivity for subunit c of mitochondrial adenosine triphosphate synthase was not observed. Based on these findings, the animal was diagnosed as affected by neuronal ceroid-lipofuscinosis. |
| Databáze: | OpenAIRE |
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