Characterisation and transferability of transcriptomic microsatellite markers for Nothofagus species
| Autor: | María Gabriela López, Maria Florencia Pomponio, Susana Torales, Sabrina Amalfi, Veronica El Mujtar, Susana N. Marcucci Poltri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nothofagus Otras Biotecnología Agropecuaria biology Biotecnología Agropecuaria Transferability MICROSATELLITES DISCRIMINANT MARKERS NOTHOFAGUS OBLIQUA Plant Science biology.organism_classification CROSS-AMPLIFICATION 03 medical and health sciences 030104 developmental biology CIENCIAS AGRÍCOLAS Botany Microsatellite Nothofagus obliqua NOTHOFAGUS ALPINA Cross amplification Ecology Evolution Behavior and Systematics |
| Zdroj: | New Zealand Journal of Botany. 55:347-356 |
| ISSN: | 1175-8643 0028-825X |
| DOI: | 10.1080/0028825x.2017.1340310 |
| Popis: | Discriminant molecular markers are required for research on population genetics, as well as evolutionary studies involving identification of hybrids and parental species, or detection of the genome regions under selection. We provide a set of 27 transcriptomic microsatellite markers (SSRs) for South American Nothofagus species, derived from 73 Nothofagus alpina (=N. nervosa) annotated unigenes. Rates of cross-amplification ranged from 22% to 37%. Genetic characterisation of 22 transcriptomic SSRs for N. alpina and N. obliqua reveals low genetic variability, due to the general occurrence of one major allele at each locus, and high specificity, with few alleles shared between species (14%). At inter-species level 95% of loci were discriminant, with a total G''st over loci of 0.9, indicating that alleles were mostly fixed for all loci in both species. At intra-species level the number of markers with significant differentiation was 2.5 times higher for N. obliqua than for N. alpina populations. Moreover, transcriptomic SSRs showed higher performance compared with published anonymous microsatellites isolated from genome sequences without annotation. This set of transcriptomic microsatellites will be useful to the scientific community working on conservation and evolutionary aspects of Nothofagus species. Fil: El Mujtar, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: López, María Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pomponio, María Florencia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina Fil: Marcucci Poltri, Susana Noemí. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torales, Susana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina |
| Databáze: | OpenAIRE |
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