Performance of screening for aneuploidies by cell-free DNA analysis of maternal blood in twin pregnancies
| Autor: | H. Dierickx, Vincenzo Cirigliano, M. M. Gil, K. H. Nicolaides, Elisa Bevilacqua, Elena Ordoñez, Jacques Jani, P. J. Willems |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty aneuploidies Gestational Age Trisomy cell-free DNA screening tests trisomies twin pregnancies Logistic regression Risk Assessment Sensitivity and Specificity Pregnancy Prenatal Diagnosis medicine Humans Pregnancy-Associated Plasma Protein-A Radiology Nuclear Medicine and imaging Chorionic Gonadotropin beta Subunit Human False Positive Reactions Prospective Studies Twin Pregnancy Gynecology Fetus Framingham Risk Score Radiological and Ultrasound Technology Cell-Free System Obstetrics business.industry Infant Newborn Obstetrics and Gynecology General Medicine DNA medicine.disease Reproductive Medicine Cell-free fetal DNA Pregnancy Twin Gestation Female business Maternal Age |
| Popis: | Objectives To report clinical implementation of cell-free DNA (cfDNA) analysis of maternal blood in screening for trisomies 21, 18 and 13 in twin pregnancies and examine variables that could influence the failure rate of the test. Methods cfDNA testing was performed in 515 twin pregnancies at 10–28 weeks' gestation. The failure rate of the test to provide results was compared with that in 1847 singleton pregnancies, and logistic regression analysis was used to determine which factors among maternal and pregnancy characteristics were significant predictors of test failure. Results Failure rate of the cfDNA test at first sampling was 1.7% in singletons and 5.6% in twins. Of those with a test result, the median fetal fraction in twins was 8.7% (range, 4.1–30.0%), which was lower than that in singletons (11.7% (range, 4.0–38.9%)). Multivariable regression analysis demonstrated that twin pregnancy, higher maternal weight and conception by in-vitro fertilization provided significant independent prediction of test failure. Follow-up was available in 351 (68.2%) of the twin pregnancies and comprised 334 with euploid fetuses, 12 discordant for trisomy 21 and five discordant for trisomy 18. In all 323 euploid cases with a result, the risk score for each trisomy was |
| Databáze: | OpenAIRE |
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