Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
| Autor: | Florent Ginhoux, Jin-Soo Kim, Young-Tae Chang, Aymeric Silvin, Sungjin Park, H. Shawn Je, Masahiro Fukuda, Audrey Tze Ting Khoo, Xiao Liu, Beomsue Kim, Sejong Choi, Taejoon Kwon, Jungyeol Lee, Xiaogang Liu, Dongdong Su, Weijie Chi, Diana S. Y. Wan, Srikanta Sanu |
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| Rok vydání: | 2019 |
| Předmět: |
Genetically modified mouse
Brain development Microglia 010405 organic chemistry Chemistry Optical Imaging Chemical probe General Chemistry 010402 general chemistry 01 natural sciences Fluorescence Catalysis In vitro 0104 chemical sciences Mice medicine.anatomical_structure nervous system In vivo medicine Animals Macrophage Glucuronosyltransferase Neuroscience Fluorescent Dyes |
| Zdroj: | Angewandte Chemie International Edition. 58:7972-7976 |
| ISSN: | 1521-3773 1433-7851 |
| Popis: | Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence. |
| Databáze: | OpenAIRE |
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