Influence of polycyclic aromatic hydrocarbon exposure on IVF: now is the time to focus on women

Autor: Blandine Courbiere, Virginie Tassitro, I. Sari-Minodier, Nicolas Beauval, Antoine Netter, Elena Siri, Noémie Resseguier, Jeanne Perrin
Přispěvatelé: Department of Gynecology, Obstetrics and Reproductive Medicine, AP-HM La Conception, Pôle femmes parents enfants, 147 bd Baille, Marseille 13005, France, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Laboratoire de santé publique (EA 3279) (public health laboratory), faculté de Médecine de la Timone, 27, boulevard Jean Moulin, Marseille cedex 5 13385, France, CHU Lille, Unité Fonctionnelle de Toxicologie, Lille F-59000, France, Service hospitalo-universitaire de médecine et santé au travail, Faculté des Sciences Médicales et Paramédicales, AP-HM La Timone, Aix Marseille Univ 27, boulevard Jean Moulin, Marseille Cedex 5 13385, France, Laboratoire de Biologie de la Reproduction - CECOS, Pole Femmes-Parents-Enfants, AP-HM La Conception, Marseille, France, Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
endocrine system
Arbitrary unit
Urinary system
Fertilization in Vitro
Urine
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Semen collection
Andrology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Pregnancy
Humans
Medicine
Polycyclic Aromatic Hydrocarbons
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
Creatinine
Granulosa Cells
Pyrenes
030219 obstetrics & reproductive medicine
business.industry
urogenital system
Obstetrics and Gynecology
Environmental Exposure
Spermatozoa
[SDE.ES]Environmental Sciences/Environmental and Society
Embryo transfer
3. Good health
Comet assay
030104 developmental biology
Reproductive Medicine
chemistry
Female
business
DNA Damage
Developmental Biology
Cohort study
Zdroj: 2020, 41 (2), pp.161-169. ⟨10.1016/j.rbmo.2020.03.022⟩
Reproductive BioMedicine
Reproductive BioMedicine Online, Elsevier, 2020, 41 (2), pp.161-169. ⟨10.1016/j.rbmo.2020.03.022⟩
ISSN: 1472-6491
DOI: 10.1016/j.rbmo.2020.03.022⟩
Popis: Research question Is polycyclic aromatic hydrocarbon (PAH) exposure associated with the reproductive outcomes of IVF treatment? Design A prospective, small-scale monocentric cohort study of couples who underwent IVF treatment between January 2018 and June 2019. Both members of each couple answered a questionnaire on PAH exposure and provided urine samples to measure urinary 1-hydroxypyrene (1-OHP) the day before oocyte retrieval and semen collection for fertilization. To assess the specific PAH exposure of gamete cells, immunostaining was conducted on both spermatozoa and granulosa cells obtained during IVF with an anti-benzo(a)pyrene diol epoxide (BPDE) monoclonal antibody that recognizes BDPE-DNA adducts. To assess DNA damage, a comet assay on spermatozoa was conducted. The PAH exposure was compared between couples who had positive HCG and couples who had negative HCG on day 14 after embryo transfer. Results Eighteen couples were included. The mean 1-OHP level in women whose HCG tests were positive (n = 6) was significantly lower than that in women with negative HCG tests (0.098 [0.042–0.170] versus 0.177 [0.067–0.812] μg/g creatinine; P = 0.048). The presence of BPDE-DNA adducts in granulosa cells of women with a negative (29.7 [16.2–57.5] arbitrary units) or positive HCG test (20.3 [9.3–23.3] arbitrary units) were not significantly different (P = 0.092). The urinary 1-OHP levels of men and BPDE-DNA adducts in spermatozoa showed no differences between groups. Conclusions This exploratory research should encourage further studies to determine the effect of women's exposure to PAHs on reproductive outcomes of IVF treatment.
Databáze: OpenAIRE
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