Residue Ionization in LpxC Directly Observed by 67Zn NMR Spectroscopy
| Autor: | Paul D. Ellis, Carol A. Fierke, Robert W. Heck, Marcy Hernick, Andrew S. Lipton |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Aquifex aeolicus biology Chemistry Analytical chemistry Active site Substrate (chemistry) Protonation General Chemistry Nuclear magnetic resonance spectroscopy biology.organism_classification Biochemistry Article Catalysis Amidohydrolases Ion NMR spectra database Kinetics Colloid and Surface Chemistry Models Chemical Ionization biology.protein Quantum Theory Zinc Isotopes Nuclear Magnetic Resonance Biomolecular |
| Zdroj: | Journal of the American Chemical Society. 130:12671-12679 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja801776c |
| Popis: | The pH dependence of the solid-state (67)Zn NMR lineshapes has been measured for both the wild type (WT) and the H265A mutant of Aquifex aeolicus LpxC, each in the absence of substrate (resting state). The (67)Zn NMR spectrum of WT LpxC at pH 6 (prepared at 0 degrees C) contains two overlapping quadrupole lineshapes with C q values of 10 and 12.9 MHz, while the spectrum measured for the sample prepared at a pH near 9 (at 0 degrees C) is dominated by the appearance of a third species with a C q of 14.3 MHz. These findings are consistent with the two p K a values previously observed by the bell-shaped dependence of the LpxC-catalyzed reaction. On the basis of comparison of the experimental results with predictions from quantum mechanical/molecular mechanical (QM/MM) modeling, we suggest that p K a1 (low pH) represents the ionization of Glu78 and p K a2 (high pH) reflects the ionization of another active site residue located near the zinc ion, such as His265. These results are also consistent with water being bound to the Zn (2+) ion throughout this pH range. The (67)Zn NMR spectra of the H265A mutant appear to be pH independent, with a C q of 9.55 MHz being sufficient to describe both low- and high-pH data. The QM/MM models of the H265A mutant suggest that over this pH range water is bound to the zinc ion while Glu78 is protonated. |
| Databáze: | OpenAIRE |
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