An Epstein-Barr virus-transformed B cell line produces autoregulatory interleukin-1 that regulates bone remodeling
| Autor: | Shunichi Hirose, Mithuru Takada, Shigeru Amano, Teturo Honda, Akiei Hamano, Shigemasa Hanazawa, Hideo Katoh, Shigeo Kitano |
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| Rok vydání: | 1989 |
| Předmět: |
Herpesvirus 4
Human medicine.medical_treatment Biology Bone resorption Bone remodeling medicine Chemical Precipitation Humans Bone Resorption Molecular Biology Cell Line Transformed Antiserum B-Lymphocytes Osteoblasts Cell growth Immune Sera Interleukin Cell Biology DNA Alkaline Phosphatase Cell Transformation Viral Chromatography Ion Exchange Molecular biology Recombinant Proteins Cytokine Biochemistry Cell culture Ammonium Sulfate Chromatography Gel Alkaline phosphatase Collagen Cell Division Interleukin-1 |
| Zdroj: | Biochimica et biophysica acta. 1012(1) |
| ISSN: | 0006-3002 |
| Popis: | In this study, we demonstrate that an Epstein-Barr virus-transformed B cell line, A-11, produced interleukin-1 (IL-1), a cytokine that regulates bone remodeling. A-11 cells produce IL-1 in a cell dose- and culture time-related manner. The IL-1 activity was neutralized by recombinant human IL-1 (rhIL-1) alpha antiserum, but not by rhIL-1 beta antiserum. The IL-1 was semi-purified by (NH4)2SO4 precipitation, Superose prep 12 gel filtration, and anion-exchange chromatography strongly stimulated in vitro bone resorption. The stimulatory effect of the purified IL-1 on bone resorption was prostaglandin independent. Purified IL-1 inhibited DNA and collagen synthesis in the osteoblastic cell line MC3T3-E1. However, it enhanced significantly the cellular activity of alkaline phosphatase (EC 3.1.3.1), a marker enzyme for differentiation of osteoblasts. On the other hand, A-11 cell proliferation was inhibited by addition of rhIL-1 alpha antiserum, but not by rhIL-1 beta antiserum. And cell proliferation was stimulated by exogenous rhIL-1 alpha and -beta. |
| Databáze: | OpenAIRE |
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