Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae Strain RS-2
| Autor: | Yang Zhang, Yushi Fang, Guochang Sun, Md. Mahidul Islam Masum, Jie Chen, Wen Qiu, Yanli Wang, Bin Li, Yingzi Yang, Ogunyemi Solabomi Olaitan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Iron-Sulfur Proteins gene knock-out Mutant Acidovorax avenae subsp. Avenae biofilm lcsh:Chemistry Gene cluster pathogenicity Pathogen lcsh:QH301-705.5 Spectroscopy Virulence Acidovorax Hcp General Medicine Type VI Secretion Systems Computer Science Applications Anti-Bacterial Agents Complementation T6SS motility Multigene Family growth 030106 microbiology Biology Catalysis Article Microbiology Inorganic Chemistry Comamonadaceae 03 medical and health sciences Bacterial Proteins Secretion Physical and Theoretical Chemistry Molecular Biology Type VI secretion system Plant Diseases Organic Chemistry Genetic Complementation Test Oryza Gene Expression Regulation Bacterial biology.organism_classification Mutagenesis Insertional lcsh:Biology (General) lcsh:QD1-999 Biofilms Genome Bacterial |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 18, Iss 10, p 2024 (2017) International Journal of Molecular Sciences; Volume 18; Issue 10; Pages: 2024 |
| ISSN: | 1422-0067 |
| Popis: | The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis. Here we compared the virulence-related phenotypes between the wild-type (RS-2) and 25 T6SS mutants, which were constructed using homologous recombination methods. The mutation of 15 T6SS genes significantly reduced bacterial virulence and the secretion of Hcp protein. Additionally, the complemented 7 mutations ΔpppA, ΔclpB, Δhcp, ΔdotU, ΔicmF, ΔimpJ, and ΔimpM caused similar virulence characteristics as RS-2. Moreover, the mutant ΔpppA, ΔclpB, ΔicmF, ΔimpJ and ΔimpM genes caused by a 38.3~56.4% reduction in biofilm formation while the mutants ΔpppA, ΔclpB, ΔicmF and Δhcp resulted in a 37.5~44.6% reduction in motility. All together, these results demonstrate that T6SS play vital roles in the virulence of strain RS-2, which may be partially attributed to the reductions in Hcp secretion, biofilm formation and motility. However, differences in virulence between strain RS-1 and RS-2 suggest that other factors may also be involved in the virulence of Aaa. |
| Databáze: | OpenAIRE |
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