In Vivo Anastomosis and Perfusion of a Three-Dimensionally-Printed Construct Containing Microchannel Networks
| Autor: | George Hung, Anderson H. Ta, Ann C. Gaffey, Jason J. Han, Samantha J. Paulsen, Patrick V. Dinh, Renganaden Sooppan, Jordan S. Miller, Alen Trubelja, Chantel M. Venkataraman, Pavan Atluri |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Materials science Laser Doppler Imaging Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Femoral artery Article Blood Vessel Prosthesis Implantation 03 medical and health sciences Tissue engineering Ischemia Blood vessel prosthesis In vivo medicine.artery medicine Animals Vascular Patency Inosculation Rats Wistar Microvessel Anastomosis Surgical Equipment Design 021001 nanoscience & nanotechnology Blood Vessel Prosthesis Hindlimb Rats Equipment Failure Analysis Femoral Artery Treatment Outcome 030104 developmental biology Printing Three-Dimensional Reperfusion 0210 nano-technology Blood Flow Velocity Biomedical engineering |
| Zdroj: | ResearcherID |
| ISSN: | 1937-3392 1937-3384 |
| Popis: | The field of tissue engineering has advanced the development of increasingly biocompatible materials to mimic the extracellular matrix of vascularized tissue. However, a majority of studies instead rely on a multiday inosculation between engineered vessels and host vasculature rather than the direct connection of engineered microvascular networks with host vasculature. We have previously demonstrated that the rapid casting of three-dimensionally-printed (3D) sacrificial carbohydrate glass is an expeditious and a reliable method of creating scaffolds with 3D microvessel networks. Here, we describe a new surgical technique to directly connect host femoral arteries to patterned microvessel networks. Vessel networks were connected in vivo in a rat femoral artery graft model. We utilized laser Doppler imaging to monitor hind limb ischemia for several hours after implantation and thus measured the vascular patency of implants that were anastomosed to the femoral artery. This study may provide a method to overcome the challenge of rapid oxygen and nutrient delivery to engineered vascularized tissues implanted in vivo. |
| Databáze: | OpenAIRE |
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