ARHGAP10 is necessary for alpha-catenin recruitment at adherens junctions and for Listeria invasion
| Autor: | Sandra Maria Zákia Lian Sousa, Didier Cabanes, Cristel Archambaud, Stéphanie Boisson-Dupuis, Marc Lecuit, Michel R. Popoff, Emmanuel Lemichez, Pascale Cossart, Edith Gouin, Pierre Legrain, Frédéric Colland |
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| Přispěvatelé: | Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Molecular Microbiology, Institute for Molecular and Cell Biology, Hybrigenics [Paris], Hybrigenics, Toxines bactériennes dans la relation hôtes-pathogènes, Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM), Bactéries anaérobies et Toxines, Institut Pasteur [Paris], Centre d'infectiologie Necker-Pasteur [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Service Hospitalier Frédéric Joliot (SHFJ), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Lemichez, Emmanuel, Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR50-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut Pasteur [Paris]-CHU Necker - Enfants Malades [AP-HP], Interactions Bactéries-Cellules ( UIBC ), Institut National de la Recherche Agronomique ( INRA ) -Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -IFR50-Institut National de la Santé et de la Recherche Médicale ( INSERM ), CHU Necker - Enfants Malades [AP-HP]-Assistance publique - Hôpitaux de Paris (AP-HP), Département de Biologie Joliot-Curie, Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) |
| Rok vydání: | 2005 |
| Předmět: |
MESH : Cell Line
RHOA MESH : Hela Cells MESH : Actins Arp2/3 complex MESH: GTPase-Activating Proteins MESH: Listeria monocytogenes Ligands Cell junction Cell membrane [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology MESH: Ligands MESH : Bacterial Proteins MESH: Animals MESH: Bacterial Proteins MESH : Ligands 0303 health sciences biology 030302 biochemistry & molecular biology GTPase-Activating Proteins MESH: Research Support N Adherens Junctions Cell biology medicine.anatomical_structure [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Intercellular Junctions Ectodomain MESH : Intercellular Junctions RNA Interference MESH: Caco-2 Cells MESH : Cell Membrane TWO-HYBRID METHOD MESH: RNA Interference ARHGAP10 Alpha catenin [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Actins ACTIN MESH: Cell Adhesion Cell Line Adherens junction 03 medical and health sciences Bacterial Proteins Two-Hybrid System Techniques medicine Cell Adhesion Animals Humans [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology [SDV.BC] Life Sciences [q-bio]/Cellular Biology ADHERENS JUNCTION 030304 developmental biology MESH: Humans [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology MESH : Humans Cell Membrane MESH : Caco-2 Cells MESH : GTPase-Activating Proteins RELATION HOTE-BACTERIE Cell Biology MESH : Adherens Junctions Actin cytoskeleton Listeria monocytogenes Actins MESH: Cell Line MESH: Hela Cells MESH : Cell Adhesion MESH: Adherens Junctions MICROBIAL INFECTION STRATEGY biology.protein MESH : Listeria monocytogenes MESH : Animals MESH : RNA Interference LIGAND Caco-2 Cells rhoA GTP-Binding Protein MESH : Research Support N alpha Catenin MESH: Intercellular Junctions MESH: Cell Membrane HeLa Cells |
| Zdroj: | Nature Cell Biology Nature Cell Biology, Nature Publishing Group, 2005, 7, pp.954-60. ⟨10.1038/ncb1308⟩ Nature Cell Biology, 2005, 7 (10), pp.954-60. ⟨10.1038/ncb1308⟩ Nature Cell Biology, Nature Publishing Group, 2005, 7 (10), pp.954-60. ⟨10.1038/ncb1308⟩ Nature Cell Biology, Nature Publishing Group, 2005, 7, pp.954-60. 〈10.1038/ncb1308〉 |
| ISSN: | 1465-7392 1476-4679 |
| DOI: | 10.1038/ncb1308⟩ |
| Popis: | E-cadherin mediates the formation of adherens junctions between epithelial cells. It serves as a receptor for Listeria monocytogenes, a bacterial pathogen that enters epithelial cells. The L. monocytogenes surface protein, InlA, interacts with the extracellular domain of E-cadherin. In adherens junctions, this ectodomain is involved in homophilic interactions whereas the cytoplasmic domain binds beta-catenin, which then recruits alpha-catenin. alpha-catenin binds to actin directly, or indirectly, thus linking E-cadherin to the actin cytoskeleton. Entry of L. monocytogenes into cells and adherens junction formation are dynamic events that involve actin and membrane rearrangements. To understand these processes better, we searched for new ligands of alpha-catenin. Using a two-hybrid screen, we identified a new partner of alpha-catenin: ARHGAP10. This protein colocalized with alpha-catenin at cell-cell junctions and was recruited at L. monocytogenes entry sites. In ARHGAP10-knockdown cells, L. monocytogenes entry and alpha-catenin recruitment at cell-cell contacts were impaired. The GAP domain of ARHGAP10 has GAP activity for RhoA and Cdc42. Its overexpression disrupted actin cables, enhanced alpha-catenin and cortical actin levels at cell-cell junctions and inhibited L. monocytogenes entry. Altogether, our results show that ARHGAP10 is a new component of cell-cell junctions that controls alpha-catenin recruitment and has a key role during L. monocytogenes uptake. |
| Databáze: | OpenAIRE |
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