| Autor: |
M. D. C. Vancetto, S. M. A. Prado, Josefina Farina Morais, Marco Antonio Stephano, Hisako Gondo Higashi, E. H. Sakaguti, F Fratelli, Rosalvo Guidolin |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Journal of Venomous Animals and Toxins, Volume: 4, Issue: 1, Pages: 70-78, Published: 1998 |
| ISSN: |
0104-7930 |
| DOI: |
10.1590/s0104-79301998000100007 |
| Popis: |
The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animals had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by enzymatic digestion and ammonium sulphate precipitation. These experiments lead us to conclude that Bothrops envenomation therapy can be successfully performed using anti-Bothrops-Tetanus serum also serving as Tetanus prophylaxis. Anti-Crotalus-Tetanus serum can also be produced, although it is not of medical interest as Crotalus envenomation rarely results in local necrotizing lesions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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