Comparison of the methods for measuring the Ki-67 labeling index in adrenocortical carcinoma: manual versus digital image analysis

Autor: Satoshi Baba, Yutaka Oki, Yukiko Shibahara, Hironobu Sasano, Ryo Morimoto, Sanae Midorikawa, Fumie Kubota-Nakayama, Yasuhiro Nakamura, Sachiko Konosu-Fukaya, Fumitoshi Satoh, Naomi Sato, Yuto Yamazaki
Rok vydání: 2016
Předmět:
Adult
Male
0301 basic medicine
Pathology
medicine.medical_specialty
Time Factors
Labeling index
Kaplan-Meier Estimate
Disease-Free Survival
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Japan
Predictive Value of Tests
Image Interpretation
Computer-Assisted
Adrenocortical Carcinoma
Humans
Medicine
Adrenocortical carcinoma
Nuclear atypia
Child
WEISS Criteria
Aged
Cell Proliferation
Neoplasm Staging
Automation
Laboratory
Observer Variation
Microscopy
biology
business.industry
Infant
Newborn
Reproducibility of Results
Middle Aged
medicine.disease
Immunohistochemistry
Adrenal Cortex Neoplasms
Ki-67 Antigen
Treatment Outcome
030104 developmental biology
030220 oncology & carcinogenesis
Predictive value of tests
Ki-67
Digital image analysis
biology.protein
Female
business
Nuclear medicine
Zdroj: Human Pathology. 53:41-50
ISSN: 0046-8177
Popis: Adrenocortical carcinoma (ACC) is a rare, highly malignant neoplasm harboring marked histologic heterogeneity. The Ki-67 labeling index (LI) is one of the most effective diagnostic and prognostic markers in ACC. However, its assessment has by no means been standardized. Therefore, in this study, we analyzed the Ki-67 LI in 18 ACC cases both by seven pathologists using microscopes (MA; manual analysis) and with digital image analysis (DIA) and also compared the Ki-67 LI obtained by selecting "hot spots" and formulating the "average" reading of the whole tumor specimen. In addition, we performed statistical analysis of the association between Ki-67 LI and the clinical and pathologic features of individual cases. The DIA was significantly correlated with MA in hot spots but not in the average fields. The Ki-67 LI in hot spots was significantly and consistently higher than that in average areas by both MA and DIA, indicating intratumoral heterogeneity. The Ki-67 LI was significantly correlated with the Weiss criteria (eosinophilic cytoplasm, nuclear atypia, atypical mitoses, and sinusoidal invasion) by any mode of evaluation. The clinical outcome was significantly better in the patients with a Ki-6710% than in those with a Ki-6710% by MA in hot spots. The Ki-67 LI in hot spots measured by MA best reflected the clinical and pathologic features of ACC. Employment of DIA to obtain the Ki-67 LI in ACC requires further improvement, including correction of its overestimation of the value by counting non-tumorous cells and nuclear segmentation in areas of high cell density.
Databáze: OpenAIRE
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