Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite
| Autor: | Limin Zhao, Xu Zhang, Xiangdong Wang, Luo Zhang, Feng Lan, Claus Bachert, Qiqi Wang, Sun Ying, Nan Zhang, Su Duan, Ming Zheng, Yuling Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Allergy Th2 response Immunology Mugwort pollen macromolecular substances medicine.disease_cause Immunoglobulin E Mugwort Cross-reactivity Peripheral blood mononuclear cell Ige binding House dust mite 03 medical and health sciences 0302 clinical medicine Immunology and Allergy Medicine 030223 otorhinolaryngology Molecular Biology biology business.industry Chemistry Research medicine.disease biology.organism_classification Tropomyosin Basophil activation 030228 respiratory system biology.protein business lcsh:RC581-607 |
| Zdroj: | Clinical and Molecular Allergy, Vol 19, Iss 1, Pp 1-9 (2021) Clinical and Molecular Allergy : CMA |
| ISSN: | 1476-7961 |
| Popis: | Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively. Results Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5. Conclusions Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation. |
| Databáze: | OpenAIRE |
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