Mutational Mapping of RAS-Responsive Domains of the Saccharomyces cerevisiae Adenylyl Cyclase
| Autor: | John J. Colicelli, Michael Wigler, Jeffrey Field, R. Ballester, Dallan Young, N. Chester |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
G protein
Genes Fungal Restriction Mapping Saccharomyces cerevisiae Biology medicine.disease_cause ADCY10 Fungal Proteins Adenylyl cyclase chemistry.chemical_compound GTP-Binding Proteins Escherichia coli medicine Cloning Molecular DNA Fungal Molecular Biology ADCY6 Mutation ADCY5 ADCY9 Cell Biology ADCY3 Molecular biology Molecular Weight chemistry ras Proteins Adenylyl Cyclases Plasmids Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.10.6.2539-2543.1990 |
| Popis: | Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not. |
| Databáze: | OpenAIRE |
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