Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model
| Autor: | Maria Ballester, Fernando Rodriguez, Júlia Vergara-Alert, Joaquim Segalés, Núria Busquets, Ayub Darji, Natàlia Majó, David Solanes, Gerard E. Martín-Valls, Veljko Veljkovic, Jordi Argilaguet, Sergio López-Soria, Raquel Rivas |
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| Rok vydání: | 2012 |
| Předmět: |
Swine
viruses T-Lymphocytes Immunofluorescence lcsh:Medicine Hemagglutinin Glycoproteins Influenza Virus Adaptive Immunity medicine.disease_cause Antibodies Viral Virus Replication Global Health Bronchoalveolar Lavage Madin Darby Canine Kidney Cells Influenza A Virus H1N1 Subtype Antibody Specificity Influenza A virus lcsh:Science Immune Response Conserved Sequence 0303 health sciences Immunity Cellular Vaccines Synthetic Multidisciplinary biology Viral Vaccine virus diseases Animal Models Immunizations 3. Good health Veterinary Diseases Medicine Research Article Influenza vaccine Immunology Hemagglutinin (influenza) Antigenic drift Virus Microbiology 03 medical and health sciences Dogs Model Organisms Species Specificity medicine Animals Humans Immunoassays Pandemics Biology Immunity to Infections 030304 developmental biology Influenza A Virus H5N1 Subtype 030306 microbiology Influenza A Virus H3N2 Subtype lcsh:R Immunity Viral Vaccines Immune Defense Veterinary Virology Virology Influenza A virus subtype H5N1 Peptide Fragments Immunity Humoral Viral replication Humoral Immunity biology.protein Immunologic Techniques Swine H1N1 lcsh:Q Immunization Veterinary Science Influenza virus |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona PLoS ONE PLOS One PLoS ONE, Vol 7, Iss 7, p e40524 (2012) |
| Popis: | Outbreaks involving either H5N1 or H1N1 influenza viruses (IV) have recently become an increasing threat to cause potential pandemics. Pigs have an important role in this aspect. As reflected in the 2009 human H1N1 pandemia, they may act as a vehicle for mixing and generating new assortments of viruses potentially pathogenic to animals and humans. Lack of universal vaccines against the highly variable influenza virus forces scientists to continuously design vaccines à la carte, which is an expensive and risky practice overall when dealing with virulent strains. Therefore, we focused our efforts on developing a broadly protective influenza vaccine based on the Informational Spectrum Method (ISM). This theoretical prediction allows the selection of highly conserved peptide sequences from within the hemagglutinin subunit 1 protein (HA1) from either H5 or H1 viruses which are located in the flanking region of the HA binding site and with the potential to elicit broader immune responses than conventional vaccines. Confirming the theoretical predictions, immunization of conventional farm pigs with the synthetic peptides induced humoral responses in every single pig. The fact that the induced antibodies were able to recognize in vitro heterologous influenza viruses such as the pandemic H1N1 virus (pH1N1), two swine influenza field isolates (SwH1N1 and SwH3N2) and a H5N1 highly pathogenic avian virus, confirm the broad recognition of the antibodies induced. Unexpectedly, all pigs also showed T-cell responses that not only recognized the specific peptides, but also the pH1N1 virus. Finally, a partial effect on the kinetics of virus clearance was observed after the intranasal infection with the pH1N1 virus, setting forth the groundwork for the design of peptide-based vaccines against influenza viruses. Further insights into the understanding of the mechanisms involved in the protection afforded will be necessary to optimize future vaccine formulations. |
| Databáze: | OpenAIRE |
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