Nrf2 Pathway Ameliorates Bladder Dysfunction in Cyclophosphamide-Induced Cystitis via Suppression of Oxidative Stress
| Autor: | Zhongqing Wei, Bin Ni, Yunpeng Shao, Baixin Shen, Le Shu, Zhengsen Chen, Yi Huang, Nebiyu Elias Tamrat |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Aging Antioxidant Article Subject Cyclophosphamide NF-E2-Related Factor 2 Urinary system medicine.medical_treatment Urinary Bladder 030232 urology & nephrology Pharmacology Protective Agents urologic and male genital diseases medicine.disease_cause environment and public health digestive system Biochemistry Antioxidants 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cystitis Animals Medicine Sensitization Gene knockout QH573-671 business.industry Interstitial cystitis Cell Biology General Medicine respiratory system medicine.disease Mice Inbred C57BL Disease Models Animal Oxidative Stress 030104 developmental biology medicine.anatomical_structure Cytology business Oxidation-Reduction Oxidative stress Research Article medicine.drug |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Objective. To investigate the protective effect and molecular mechanism of nuclear factor E2-related factor 2 (Nrf2) pathway in interstitial cystitis (IC). Methods. We established a mouse model of IC by cyclophosphamide (CYP) in wild-type mice and Nrf2 gene knockout mice. We examined the histological and functional alterations, the changes of oxidative stress markers, and the expression of the antioxidant genes downstream of Nrf2 pathway. Results. After CYP administration, the mice showed urinary frequency and urgency, pain sensitization, decreased contractility, bladder edema, and oxidative stress disorder. Notably, the Nrf2-/- CYP mice had more severe symptoms. The mRNA and protein levels of antioxidant genes downstream of Nrf2 pathway were significantly upregulated in the Nrf2+/+ CYP mice, while there were no significant changes in the Nrf2-/- CYP mice. Conclusion. Nrf2 pathway protects bladder injury and ameliorates bladder dysfunction in IC, possibly by upregulating antioxidant genes and inhibiting oxidative stress. |
| Databáze: | OpenAIRE |
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