Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles
| Autor: | Veronika Kralj-Iglič, Manca Pajnič, Vid Šuštar, Barbara Drasler, Henry Hägerstrand, Judita Lea Krek, Roman Štukelj, Darko Makovec, Veno Kononenko, Metka Šimundić, Damjana Drobne |
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| Jazyk: | angličtina |
| Předmět: |
Adult
Blood Platelets Male Blood cells Echinocyte Air pollution Biomedical Engineering Carbon black nanomaterial Medicine (miscellaneous) Pharmaceutical Science Bioengineering Buffers Applied Microbiology and Biotechnology Blood cell Cell membrane Phospholipid bilayer Soot Suspensions Carbon black Blood plasma medicine Model systems Humans Nanotoxicity Lipid bilayer Cell Shape Phospholipids Chemistry Vesicle Research Cell Membrane Erythrocyte Membrane Biological membrane Nanostructures Cell membranes Membrane medicine.anatomical_structure Biochemistry Biophysics Microscopy Electron Scanning Molecular Medicine Female |
| Zdroj: | Journal of Nanobiotechnology |
| ISSN: | 1477-3155 |
| DOI: | 10.1186/s12951-015-0087-3 |
| Popis: | Background We studied the effect of carbon black (CB) agglomerated nanomaterial on biological membranes as revealed by shapes of human erythrocytes, platelets and giant phospholipid vesicles. Diluted human blood was incubated with CB nanomaterial and observed by different microscopic techniques. Giant unilamellar phospholipid vesicles (GUVs) created by electroformation were incubated with CB nanomaterial and observed by optical microscopy. Populations of erythrocytes and GUVs were analyzed: the effect of CB nanomaterial was assessed by the average number and distribution of erythrocyte shape types (discocytes, echinocytes, stomatocytes) and of vesicles in test suspensions, with respect to control suspensions. Ensembles of representative images were created and analyzed using computer aided image processing and statistical methods. In a population study, blood of 14 healthy human donors was incubated with CB nanomaterial. Blood cell parameters (concentration of different cell types, their volumes and distributions) were assessed. Results We found that CB nanomaterial formed micrometer-sized agglomerates in citrated and phosphate buffered saline, in diluted blood and in blood plasma. These agglomerates interacted with erythrocyte membranes but did not affect erythrocyte shape locally or globally. CB nanomaterial agglomerates were found to mediate attractive interaction between blood cells and to present seeds for formation of agglomerate - blood cells complexes. Distortion of disc shape of resting platelets due to incubation with CB nanomaterial was not observed. CB nanomaterial induced bursting of GUVs while the shape of the remaining vesicles was on the average more elongated than in control suspension, indicating indirect osmotic effects of CB nanomaterial. Conclusions CB nanomaterial interacts with membranes of blood cells but does not have a direct effect on local or global membrane shape in physiological in vitro conditions. Blood cells and GUVs are convenient and ethically acceptable methods for the study of effects of various substances on biological membranes and therefrom derived effects on organisms. |
| Databáze: | OpenAIRE |
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