Molecular epidemiology, antimicrobial susceptibility, and characterization of macrolide-resistant Streptococcus pyogenes in Japan
| Autor: | Yushiro Yamashita, Mariko Teramachi, Shizuo Shindou, Kenji Gotoh, Naoki Tsumura, Yuhei Tanaka, Kazuhisa Ishimoto |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Streptococcus pyogenes 030106 microbiology Microbial Sensitivity Tests Biology medicine.disease_cause Group A Microbiology 03 medical and health sciences Japan Streptococcal Infections Drug Resistance Bacterial Pulsed-field gel electrophoresis medicine Humans Pharmacology (medical) Child Respiratory Tract Infections Gel electrophoresis Antiinfective agent Antigens Bacterial Molecular Epidemiology Molecular epidemiology Macrolide resistant Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Phenotype Multilocus sequence typing Macrolides Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 22(11) |
| ISSN: | 1437-7780 |
| Popis: | Here we report the molecular epidemiology of macrolide-resistant Streptococcus pyogenes (group A streptococci, GAS) isolated from children with pharyngotonsillitis between 2011 and 2013 in Japan. In 299 isolates, 124 (41.5%) isolates were macrolide-resistant. We characterized the isolates by emm typing, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Of 299 isolates, 124 (41.5%) were macrolide-resistant isolates, 76 (61.3%) possessed mefA and 46 (37.1%) possessed ermB. All 76 isolates with mefA possessed msrD. There were no isolates possessed ermTR in this study. Eight emm/MLST types were observed. The predominant type was emm1/ST28 (57 isolates, 46.0%), which possessed the mefA/msrD complex, presenting as the M phenotype. The second most predominant type was emm12/ST467, which possessed ermB, presenting as the cMLSB phenotype. Of the cMLSB phenotype isolates, types emm28/ST52 and emm12/ST36 had multiple genetic backgrounds. We found high proportions of macrolide-resistant GAS in the southwestern areas of Japan. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect