Suppression of HCV RNA replication by baculovirus-mediated shRNA expression
| Autor: | Hitoshi Suzuki, Hiroyasu Kaneko, Naoko Miyano-Kurosaki, Nobushige Tamai, Kunitada Shimotohno, Kahoko Hashimoto, Hiroshi Takaku |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
RNA
Untranslated Viral Core Proteins Genetic Vectors virus diseases RNA-dependent RNA polymerase Hepacivirus General Medicine Biology Virus Replication Virology digestive system diseases Small hairpin RNA NS2-3 protease Viral replication DNA-directed RNA interference RNA interference Transcription (biology) RNA Viral RNA Interference Replicon Baculoviridae |
| Zdroj: | Nucleic Acids Symposium Series. 49:339-340 |
| ISSN: | 1746-8272 0261-3166 |
| Popis: | We describe a HCV core-short-hairpin RNA (shRNA) expressed from a human U6 Pol II promoter, which synergistically enhanced the inhibition of HCV replication in HCV replicon. We also describe the construction of recombinant baculovirus expressing shRNAs of the HCV core gene under the control of the U6 promoter. The anti-HCV shRNAs may provide a practical basis for applying shRNA-based gene therapy to the treatment of HCV. |
| Databáze: | OpenAIRE |
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