Detection of DNA strand breaks by comet assay in sputum leucocytes of bitumen-exposed workers: A pilot study
| Autor: | Monika Raulf-Heimsoth, Thomas Brüning, Boleslaw Marczynski, Heiko U. Käfferlein, Vosshans B, Benjamin Kendzia, Rainer Bramer, Eun-Hyun Lee, Gerda Borowitzki, Beate Pesch |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Neutrophils DNA damage Health Toxicology and Mutagenesis medicine.medical_treatment Arbitrary unit Cell Count Pilot Projects Air Pollutants Occupational Biology Toxicology medicine.disease_cause Blood cell Young Adult chemistry.chemical_compound Occupational Exposure Leukocytes medicine Humans Lymphocytes Macrophages DNA Breaks Interleukin-8 Sputum Epithelial Cells General Medicine Middle Aged Molecular biology Hydrocarbons Comet assay medicine.anatomical_structure Cytokine chemistry Immunology Irritants Comet Assay Inflammation Mediators medicine.symptom Genotoxicity DNA Mutagens |
| Zdroj: | Human & Experimental Toxicology. 29:721-729 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | DNA strand breaks were determined in leucocytes of induced sputum (IS) and compared with DNA strand breaks in blood lymphocytes from 42 bitumen-exposed workers pre and post shift. Comet assay results were expressed in arbitrary units based on visual scoring (sputum leucocytes) and Olive tail moment (OTM, blood lymphocytes). DNA damage in IS leucocytes was overall high but did not change during shift. Level of DNA strand breaks in IS samples correlated with total cell count and neutrophil content (Spearman rank correlation coefficient rs = 0.47, p = 0.001, rs= 0.48, p = 0.001, respectively) and with IL-8 concentration before and after shift (rs = 0.31, P = 0.048, and rs = 0.43, P = 0.005). DNA damage in IS was not associated with DNA strand breaks in blood lymphocytes (rs = —0.04, p = 0.802 before shift, rs = 0.27, p = 0.088 after shift). A higher level of DNA strand breaks was measured in blood lymphocytes before shift (median OTM 1.7 before and 1.3 after shift, p = 0.023). A strong correlation was found between the number of neutrophils and IL-8 concentration in IS before and after shift (rs = 0.77 and rs= 0.75, p < 0.001). This study showed an association between genotoxic and inflammatory effects in the lower airways and compared simultaneously DNA strand breaks in IS and blood of bitumen-exposed workers. |
| Databáze: | OpenAIRE |
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