Utility of Pandemic H1N1 2009 Influenza Virus Recombinant Hemagglutinin Protein-Based Enzyme-Linked Immunosorbent Assay for Serosurveillance
| Autor: | Rashmi G Virkar, Babasaheb V. Tandale, Vidya A. Arankalle, Nilesh B Ingle |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Immunology Hemagglutinins Viral Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity Virus law.invention Influenza A Virus H1N1 Subtype law Virology Influenza Human medicine Influenza A virus Humans Immunology and Allergy Seroprevalence Antigens Viral Hemagglutination assay Hemagglutination Inhibition Tests Recombinant Proteins Influenza A virus subtype H5N1 Titer Immunoglobulin G Recombinant DNA biology.protein Microbial Immunology Antibody |
| Zdroj: | Clinical and Vaccine Immunology. 17:1481-1483 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies against the pandemic H1N1 2009 influenza A virus, employing a recombinant hemagglutinin protein of the virus, was compared to the hemagglutination inhibition (HI) test using 783 serum samples. The results showed a concordance of 98.4%, suggesting the utility of the ELISA in serosurveillance. Two hundred sixty-nine (100%) serum samples with an HI titer of ≥20 were ELISA reactive. |
| Databáze: | OpenAIRE |
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