Expression and distribution of PIEZO1 in the mouse urinary tract
| Autor: | Gerard Apodaca, Marcelo D. Carattino, William A. Ricke, Dennis R. Clayton, Marianela G. Dalghi, Thomas R. Kleyman, Mohammad M. Al-bataineh, Wily G. Ruiz, Lisa M. Satlin |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Physiology Urinary system Mice Transgenic Mechanotransduction Cellular Ion Channels 03 medical and health sciences 0302 clinical medicine Genes Reporter Proper function medicine Pressure Distribution (pharmacology) Animals Tissue Distribution Mechanotransduction Urinary Tract Kidney business.industry PIEZO1 Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Urethra Gene Expression Regulation Female Stress Mechanical business 030217 neurology & neurosurgery Research Article |
| Zdroj: | Am J Physiol Renal Physiol |
| ISSN: | 1522-1466 |
| Popis: | The proper function of the organs that make up the urinary tract (kidneys, ureters, bladder, and urethra) depends on their ability to sense and respond to mechanical forces, including shear stress and wall tension. However, we have limited understanding of the mechanosensors that function in these organs and the tissue sites in which these molecules are expressed. Possible candidates include stretch-activated PIEZO channels (PIEZO1 and PIEZO2), which have been implicated in mechanically regulated body functions including touch sensation, proprioception, lung inflation, and blood pressure regulation. Using reporter mice expressing a COOH-terminal fusion of Piezo1 with the sequence for the tandem-dimer Tomato gene, we found that PIEZO1 is expressed in the kidneys, ureters, bladder, and urethra as well as organs in close proximity, including the prostate, seminal vesicles and ducts, ejaculatory ducts, and the vagina. We further found that PIEZO1 expression is not limited to one cell type; it is observed in the endothelial and parietal cells of the renal corpuscle, the basolateral surfaces of many of the epithelial cells that line the urinary tract, the interstitial cells of the bladder and ureters, and populations of smooth and striated muscle cells. We propose that in the urinary tract, PIEZO1 likely functions as a mechanosensor that triggers responses to wall tension. |
| Databáze: | OpenAIRE |
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