Transportation of de novo synthesized jasmonoyl isoleucine in tomato
| Autor: | Chikara Masuta, Chizuru Sato, Kae Sueda, Hideyuki Matsuura, Syohei Takeishi, Naoki Kiatoka, Kensuke Nabeta |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
biology
Nicotiana tabacum Jasmonic acid fungi Mutant Molecular Conformation food and beverages Cyclopentanes Plant Science General Medicine Horticulture biology.organism_classification Biochemistry Molecular biology Plant Leaves Kinetics chemistry.chemical_compound Solanum lycopersicum chemistry Isoleucine Solanum Molecular Biology Jasmonoyl-isoleucine Solanaceae |
| Zdroj: | Phytochemistry. 83:25-33 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/j.phytochem.2012.06.009 |
| Popis: | In plants, jasmonic acid (JA) and its derivatives are thought to be involved in mobile forms of defense against biotic and abiotic stresses. In this study, the distal transport of JA-isoleucine (JA-Ile) that is synthesized de novo in response to leaf wounding in tomato ( Solanum lycopersicum ) plants was investigated. JA-[ 13 C 6 ]Ile was recovered in distal untreated leaves after wounded leaves were treated with [ 13 C 6 ]Ile. However, as [ 13 C 6 ]Ile was also recovered in the distal untreated leaves, whether JA-Ile was synthesized in the wounded or in the untreated leaves was unclear. Hence, stem exudates were analyzed to obtain more detailed information. When [ 13 C 6 ]Ile and [ 2 H 6 ]JA were applied separately into the wounds on two different leaves, JA-[ 13 C 6 ]Ile and [ 2 H 6 ]JA-Ile were detected in the stem exudates but [ 2 H 6 ]JA–[ 13 C 6 ]Ile was not, indicating that JA was conjugated with Ile in the wounded leaf and that the resulting JA-Ile was then transported into systemic tissues. The [ 2 H 3 ]JA-Ile that was applied exogenously to the wounded tissues reached distal untreated leaves within 10 min. Additionally, applying [ 2 H 3 ]JA-Ile to the wounded leaves at concentrations of 10 and 60 nmol/two leaves induced the accumulation of PIN II , LAP A , and JAZ3 mRNA in the distal untreated leaves of the spr2 mutant S. lycopersicum plants. These results demonstrate the transportation of de novo synthesized JA-Ile and suggest that JA-Ile may be a mobile signal. |
| Databáze: | OpenAIRE |
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