Towards the standardization of methods of tissue processing for the isolation of mesenchymal stromal cells for clinical use
| Autor: | Elisabeth García-Muñoz, Joaquim Vives |
|---|---|
| Přispěvatelé: | Institut Català de la Salut, [García-Muñoz E] Banc de Sang i Teixits, 08005 Barcelona, Spain. [Vives J] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup d'Enginyeria de Teixits Musculoesquelètics, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Methods Paper Therapeutics::Biological Therapy::Cell- and Tissue-Based Therapy::Cell Transplantation::Stem Cell Transplantation::Mesenchymal Stem Cell Transplantation [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT] Biomedical Engineering Adipose tissue Bioengineering Sistema nerviós - Degeneració - Tractament Bioinformatics Regenerative medicine 03 medical and health sciences 0302 clinical medicine Wharton's jelly MSCs isolation protocols Cèl·lules mare mesenquimàtiques - Ús terapèutic Medicine Bone marrow enfermedades del sistema nervioso::enfermedades neurodegenerativas [ENFERMEDADES] Umbilical cord Multipotent mesenchymal stromal cells Otros calificadores::/terapia [Otros calificadores] terapéutica::terapia biológica::tratamientos basados en células y tejidos::trasplante de células::trasplante de células madre::trasplante de células madre mesenquimatosas [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS] Wharton’s jelly business.industry Mesenchymal stem cell Tissue Processing Cell Biology Other subheadings::/therapy [Other subheadings] Transplantation 030104 developmental biology medicine.anatomical_structure Nervous System Diseases::Neurodegenerative Diseases [DISEASES] 030220 oncology & carcinogenesis business Biotechnology Adult stem cell |
| Zdroj: | Scientia Cytotechnology Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| Popis: | Teixit adipós; Medul·la òssia; Cèl·lules estromals mesenquimals multipotents Tejido adiposo; Médula ósea; Células estromales mesenquimales multipotentes Adipose tissue; Bone marrow; Multipotent mesenchymal stromal cells Multipotent mesenchymal stromal cells (MSCs) are currently the most extensively studied type of adult stem cells in advanced stages of development in the field of regenerative medicine. The biological properties of MSCs have generated great hope for their therapeutic use in degenerative and autoimmune conditions that, at present, lack effective treatment options. Over the last decades, MSCs have been typically obtained from adult bone marrow, but the extraction process is highly invasive and the quality and numbers of isolated cells is drastically influenced by patient age, medication and associated comorbidities. Therefore, there is currently an open discussion on the convenience of allogeneic over autologous treatments, despite potential disadvantages such as rejection by the host. This shift to the allogeneic setting entails the need for high production of MSCs to ensure availability of sufficient cell numbers for transplantation, and therefore making the search for alternative tissue sources of highly proliferative MSC cultures with low levels of senescence occurrence, which is one of the greatest current challenges in the scale up of therapeutic cell bioprocessing. Herein we (i) present the main isolation protocols of MSCs from bone marrow, adipose tissue and Wharton’s jelly of the umbilical cord; and (ii) compare their qualities from a bioprocess standpoint, addressing both quality and regulatory aspects, in view of their anticipated clinical use. This work has been developed in the context of the Spanish Cell Therapy Network (TerCel, expedient number: RD16/0011/0028) and project PI19/01788, which is funded by Instituto de Salud Carlos III and co-funded by European Union (ERDF/ESF)—A way to build Europe. |
| Databáze: | OpenAIRE |
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