ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria
| Autor: | H.P. Schweizer, T. Hoang, Martin Handfield, François Sanschagrin, M.J. Mahan, Roger C. Levesque |
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| Rok vydání: | 1998 |
| Předmět: |
Genetic Markers
Gram-negative bacteria Genetic Vectors Green Fluorescent Proteins Mutant General Biochemistry Genetics and Molecular Biology Green fluorescent protein Microbiology Plasmid Bacterial Proteins Genes Reporter In vivo Gram-Negative Bacteria Gene expression Escherichia coli Cloning Molecular Promoter Regions Genetic Selectable marker Reporter gene Sequence Homology Amino Acid biology Aspartate-Semialdehyde Dehydrogenase Gene Expression Regulation Bacterial beta-Galactosidase biology.organism_classification Molecular biology Luminescent Proteins Lac Operon Pseudomonas aeruginosa Plasmids Biotechnology |
| Zdroj: | BioTechniques. 24:261-264 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/98242st02 |
| Popis: | We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein with enhanced fluorescence properties (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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