Epidermal growth factor regulates PAI-1 expression via activation of the transcription factor Elk-1
| Autor: | Jakub Kochan, Krystyna Stalińska, Mateusz Wawro, Aneta Kasza, Paulina Wyrzykowska |
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| Rok vydání: | 2010 |
| Předmět: |
Transcriptional Activation
animal diseases Biophysics Breast Neoplasms Biology Adenocarcinoma Transfection Biochemistry Metastasis Proto-Oncogene Protein c-ets-1 EGF signaling Breast cancer Elk-1 breast cancer Structural Biology Epidermal growth factor Plasminogen Activator Inhibitor 1 Genetics medicine Tumor Cells Cultured Humans Growth factor receptor inhibitor Binding site Phosphorylation Promoter Regions Genetic Molecular Biology Transcription factor ets-Domain Protein Elk-1 Regulation of gene expression Binding Sites Epidermal Growth Factor medicine.disease PAI promoter Gene Expression Regulation Neoplastic Cancer research Female Chromatin immunoprecipitation Protein Kinases Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 1799(9) |
| ISSN: | 0006-3002 |
| Popis: | PAI-1 (plasminogen activator inhibitor-1) in breast cancer cells is involved in tumour development and metastasis of breast cancer cells. PAI-1 function and the regulation of its expression have been precisely investigated. Here we report that EGF, which promotes breast cancer tumour growth and survival, rapidly induces PAI-1 expression in the breast adenocarcinoma cell line MCF-7 through the activation of the transcription factor Elk-1. We have found that the PAI-1 promoter fragment (-140 to +173) containing the Ets consensus binding site is activated by Elk-1. Chromatin immunoprecipitation analysis confirms in vivo binding of Elk-1 to the PAI-1 promoter and demonstrates that Elk-1 phosphorylation on the Ets binding site is EGF-dependent. |
| Databáze: | OpenAIRE |
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