Regulation of ribozyme activity by engineered protein switch
| Autor: | Yo Kikuchi, Terumichi Tanaka, Naomi Kanda |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
RNase P
Escherichia coli Proteins Protein subunit fungi Ribozyme food and beverages Heterologous General Medicine Biology medicine.disease_cause Ribonuclease P Biochemistry Gene Products tat biology.protein medicine Ribonuclease P activity Mammalian CPEB3 ribozyme Genetic Engineering Escherichia coli VS ribozyme HIV Long Terminal Repeat |
| Zdroj: | Nucleic Acids Symposium Series. 49:343-344 |
| ISSN: | 1746-8272 0261-3166 |
| DOI: | 10.1093/nass/49.1.343 |
| Popis: | Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain. |
| Databáze: | OpenAIRE |
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