Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
| Autor: | Peter C. Fineran, Rafael Pinilla-Redondo, Robert D. Fagerlund, Chris M. Brown, Nicole D. Marino, Søren J. Sørensen, Saadlee Shehreen, Joseph Bondy-Denomy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Candidate gene Pectobacterium CRISPR-Cas systems Science 030106 microbiology General Physics and Astronomy Computational biology Serratia General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Bacterial Proteins immune system diseases Extrachromosomal DNA CRISPR Bacteriophages skin and connective tissue diseases lcsh:Science Gene Multidisciplinary biology Bacteria General Chemistry Interspersed Repetitive Sequences biology.organism_classification 030104 developmental biology Multigene Family lcsh:Q Mobile genetic elements |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) Pinilla-Redondo, R, Shehreen, S, Marino, N D, Fagerlund, R D, Brown, C M, Sørensen, S J, Fineran, P C & Bondy-Denomy, J 2020, ' Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements ', Nature Communications, vol. 11, 5652 . https://doi.org/10.1038/s41467-020-19415-3 Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems. Mobile genetic elements have evolved anti-CRISPR (Acr) proteins to bypass the immunity provided by prokaryotic CRISPR–Cas systems. Here, the authors identify 11 type I Acrs encoded on mobile genetic elements, and show that acr loci neighborhoods can be used to discover inhibitors of other bacterial defense systems. |
| Databáze: | OpenAIRE |
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