Sox17 regulates proliferation and cell cycle during gastric cancer progression
| Autor: | Yan Wei Ye, Ying Qiang Shi, Bi Qiang Zheng, Jiang Hong Wu, Xi Cao, Chun Meng Wang, Meng Hong Sun, Xiao Yan Zhou, Ye Zhou, Chun Yan Du |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
animal structures Blotting Western Fluorescent Antibody Technique Biology medicine.disease_cause Polymerase Chain Reaction Stomach Neoplasms SOXF Transcription Factors medicine Humans RNA Small Interfering Clonogenic assay Cell Proliferation DNA Primers Gene knockdown Base Sequence Cell Cycle Wnt signaling pathway Cancer Transfection Cell cycle medicine.disease Molecular biology digestive system diseases Oncology Gene Knockdown Techniques embryonic structures Cancer cell Disease Progression Carcinogenesis |
| Zdroj: | Cancer Letters. 307:124-131 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2011.03.024 |
| Popis: | Sox17, a transcription factor, was considered as an antagonist to inhibit canonical Wnt/β-catenin signaling in several malignant tumors. Extremely little is known about Sox17 in gastric cancer. Therefore, the aim of this study was to elucidate the vital role of Sox17 in the tumorigenesis and progression of gastric cancer. Real time PCR was used to detect the expression of Sox17 in gastric cancer tissue. Furthermore, a series of function assays, utilizing small interfering RNA (siRNA)-mediated knockdown of Sox17 expression in MKN45 gastric cancer cells, have been performed in this study, including proliferation assay, clonogenic assay, cell-cycle evaluation, apoptosis detection as well as western blot assay. Sox17 expression were low in gastric cancer tissues as compared to normal tissue (P=0.013). Knockdown of Sox17 by Sox17-siRNA markedly enhanced the proliferation ability of MKN45 cells when compared with negative siRNA-infected cells and mock group (P |
| Databáze: | OpenAIRE |
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