E-cigarettes and Western Diet: Important Metabolic Risk Factors for Hepatic Diseases

Autor: Meher Parveen, Indrani Sinha-Hikim, Amiya P. Sinha-Hikim, Jorge Espinoza-Derout, Carl Sims, Xuesi Shao, Desean L. Lee, Theodore C. Friedman, Kamrul Hasan
Rok vydání: 2019
Předmět:
Liver Cirrhosis
0301 basic medicine
Apolipoprotein E
Male
Biopsy
Medical Biochemistry and Metabolomics
Electronic Nicotine Delivery Systems
medicine.disease_cause
Inbred C57BL
Oral and gastrointestinal
Nicotine
chemistry.chemical_compound
Mice
Random Allocation
0302 clinical medicine
Needle
methods [Real-Time Polymerase Chain Reaction]
2.1 Biological and endogenous factors
Aetiology
Cotinine
Mice
Knockout

Blotting
Liver Disease
Biopsy
Needle

blood [Cotinine]
Immunohistochemistry
030211 gastroenterology & hepatology
Western
medicine.drug
medicine.medical_specialty
Knockout
Chronic Liver Disease and Cirrhosis
Blotting
Western

Clinical Sciences
Immunology
Biology
Real-Time Polymerase Chain Reaction
Article
03 medical and health sciences
etiology [Fatty Liver]
Internal medicine
Tobacco
medicine
Animals
Humans
Protein kinase A
Nutrition
etiology [Liver Cirrhosis]
Analysis of Variance
adverse effects [Western]
Tobacco Smoke and Health
Gastroenterology & Hepatology
Hepatology
Triglyceride
Animal
Lipid metabolism
medicine.disease
Adenosine
Diet
Mice
Inbred C57BL

Fatty Liver
Disease Models
Animal

Oxidative Stress
Good Health and Well Being
030104 developmental biology
Endocrinology
chemistry
Diet
Western

Disease Models
pathology
Steatosis
Digestive Diseases
Oxidative stress
Zdroj: Hepatology (Baltimore, Md.), vol 69, iss 6
Popis: The use of electronic nicotine delivery systems (ENDS), also known as e-cigarettes, with a variety of e-liquids/e-juices, is increasing at an alarming rate among adolescents who do not realize the potential harmful health effects. This study examines the harmful effects of ENDS on the liver. Apolipoprotein E null (ApoE-/-) mice on a western diet (WD) were exposed to saline or ENDS with 2.4% nicotine aerosol for 12 weeks using our mouse ENDS exposure model system, which delivers nicotine to mice and leads to equivalent serum cotinine levels found in human cigarette users. ApoE-/- mice on a WD exposed to ENDS exhibited a marked increase in hepatic lipid accumulation compared with ApoE-/- on a similar diet exposed to saline aerosol. The detrimental effects of ENDS on hepatic steatosis were associated with significantly greater oxidative stress, increased hepatic triglyceride levels, and increased hepatocyte apoptosis, independent of adenosine monophosphate-activated protein kinase signaling. In addition, hepatic RNA sequencing analysis revealed that 433 genes were differentially expressed in ENDS-exposed mice on WD compared with saline-exposed mice. Functional analysis indicates that genes associated with lipid metabolism, cholesterol biosynthesis, and circadian rhythm were most significantly altered in the liver in response to ENDS. Conclusion: These results demonstrate profound adverse effects of ENDS on the liver. This is important information for regulatory agencies as they regulate ENDS.
Databáze: OpenAIRE