Baicalin Magnesium Salt Attenuates Lipopolysaccharide-Induced Acute Lung Injury via Inhibiting of TLR4/NF-κB Signaling Pathway

Autor: Ji-Gang Piao, Cuizhe Liu, Lizong Zhang, Hongyue Zheng, Xiaowei Xie, Fanzhu Li, Lin Zhang, Hangsheng Zheng, Lukun Yang
Rok vydání: 2021
Předmět:
Lipopolysaccharides
Male
0301 basic medicine
Article Subject
Lipopolysaccharide
Acute Lung Injury
Immunology
Pharmacology
Lung injury
medicine.disease_cause
Proinflammatory cytokine
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Humans
Immunology and Allergy
Magnesium
Lung
Flavonoids
biology
medicine.diagnostic_test
Plant Extracts
Chemistry
NF-kappa B
General Medicine
RC581-607
respiratory system
biology.organism_classification
respiratory tract diseases
Toll-Like Receptor 4
Disease Models
Animal
Oxidative Stress
030104 developmental biology
Bronchoalveolar lavage
030220 oncology & carcinogenesis
Myeloperoxidase
TLR4
biology.protein
Scutellaria baicalensis
Immunologic diseases. Allergy
Bronchoalveolar Lavage Fluid
Oxidative stress
Research Article
Signal Transduction
Zdroj: Journal of Immunology Research
Journal of Immunology Research, Vol 2021 (2021)
ISSN: 2314-7156
2314-8861
Popis: Baicalin (BA) magnesium salt (BA-Mg) is a good water-soluble ingredient extracted from Scutellaria baicalensis Georgi, a commonly used traditional Chinese medicine. This study is aimed at investigating whether BA-Mg could exert a better protective effect on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and illuminate the underlying mechanisms in vivo and in vitro. Mice were intraperitoneally administrated with equimolar BA-Mg, BA, and MgSO4 before LPS inducing ALI. Lung tissues and bronchoalveolar lavage fluid were collected for lung wet/dry ratio, histological examinations, cell counts, and biochemical analyses at 48 h post-LPS exposure. Meanwhile, the protein expressions of TLR4/NF-κB signaling pathway and proinflammatory cytokines in lung tissues and lung bronchial epithelial cells (BEAS-2B) were detected. The results showed BA-Mg pronouncedly ameliorated LPS-induced inflammatory response and histopathological damages, elevated antioxidant enzyme activity (SOD), and downregulated myeloperoxidase (MPO) and malonaldehyde (MDA) levels through the inhibition of TLR4/NF-κB signaling pathway activation. Moreover, the effect of BA-Mg was significantly better than that of BA and MgSO4 in ameliorating symptoms. Overall, BA-Mg can effectively relieve inflammatory response and oxidative stress triggered by LPS, indicating it may be a potential therapeutic candidate for treating ALI.
Databáze: OpenAIRE
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