Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid
| Autor: | Ana Lúcia da Costa Darini, Yohei Doi, Renata Galetti, Anelise Stella Ballaben, Doroti de Oliveira Garcia, Leonardo Neves Andrade, Joseane Cristina Ferreira |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Pseudomonas monteilii 030106 microbiology Virulence Drug resistance Microbial Sensitivity Tests medicine.disease_cause Integron Microbiology 03 medical and health sciences Japan Inosine Monophosphate Drug Resistance Multiple Bacterial Pseudomonas Genetics medicine Humans Pseudomonas Infections MENINGITE Molecular Biology Pathogen Ecology Evolution Behavior and Systematics Cerebrospinal Fluid biology Pseudomonas aeruginosa Sequence Analysis DNA biology.organism_classification Resistome Anti-Bacterial Agents 030104 developmental biology Infectious Diseases biology.protein Bacteria |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1567-7257 |
| Popis: | IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed blaIMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, blaIMP-16, blaOXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir. |
| Databáze: | OpenAIRE |
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