Autoantibodies to DNA-dependent protein kinase. Probes for the catalytic subunit
| Autor: | Shinichi Inada, Tsuneyo Mimori, Michito Hirakata, Yutaka Okano, A. Suwa, Yoshihiko Takeda, Hirobumi Teraoka, John A. Hardin, Fumiaki Watanabe, William S. Dynan |
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| Rok vydání: | 1996 |
| Předmět: |
Protein subunit
Cyclin-dependent kinase 2 DNA Helicases Nuclear Proteins Antigens Nuclear Enzyme-Linked Immunosorbent Assay DNA-Activated Protein Kinase General Medicine Protein Serine-Threonine Kinases Biology Molecular biology Ku Protein MAP2K7 DNA-Binding Proteins Biochemistry Cyclin-dependent kinase complex biology.protein Humans c-Raf Protein kinase A Ku Autoantigen DNA-PKcs Research Article Autoantibodies HeLa Cells |
| Zdroj: | Journal of Clinical Investigation. 97:1417-1421 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci118562 |
| Popis: | DNA-dependent protein kinase (DNA-PK) is an important nuclear enzyme which consists of a catalytic subunit known as DNA-PKcs and a regulatory component identified as the Ku autoantigen. In the present study, we surveyed 312 patients in a search for this specificity. 10 sera immunoprecipitated a large polypeptide which exactly comigrated with DNA-PKcs in SDS-PAGE. Immunoblot analysis demonstrated that this polypeptide was recognizable by a rabbit antiserum specific for DNA-PKcs. Although the patient sera did not bind to biochemically purified DNA-PKcs in immunoblots or ELISA, they were able to deplete DNA-PK catalytic activity from extracts of HeLa cells in a dose-dependent manner. We conclude that these antibodies should be useful probes for studies which aim to define the role of DNA-PK in cells. Since six sera simultaneously contained antibodies to the Ku protein, these studies suggest that relatively intact forms of DNA-PK complex act as autoantigenic particles in selected patients. |
| Databáze: | OpenAIRE |
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