The effect of melatonin on procarbazine induced testicular toxicity on rats

Autor:	Onur Erdem, Ercan Malkoç, Emin Ozgur Akgul, Oguzhan Babacan, Bilal Firat Alp, Mustafa Gülgün, Nuri Yigit, Nadir Korkmazer, Vural Kesik, Mehmet Saldir, Yavuz Poyrazoglu
Rok vydání:	2014
Předmět:	Male endocrine system medicine.medical_specialty Urology Antineoplastic Agents Testicle Biology Procarbazine Testicular Diseases Antioxidants Melatonin Malondialdehyde Internal medicine Testis medicine Animals Testosterone Rats Wistar Nitrites chemistry.chemical_classification Glutathione Peroxidase Nitrates Sertoli Cells urogenital system Glutathione peroxidase Sertoli cell Spermatozoa Sperm Disease Models Animal medicine.anatomical_structure Endocrinology Seminiferous tubule Reproductive Medicine chemistry Cytoprotection Sperm Motility Follicle Stimulating Hormone medicine.drug
Zdroj:	Systems Biology in Reproductive Medicine. 60:323-328
ISSN:	1939-6376 1939-6368
Popis:	Procarbazine (P) is an effective chemotherapeutic drug especially used in lymphoma treatment; however testicular toxicity is a limiting factor. Various ways of treatment were tried to preserve testicular function including hormonal treatment, antioxidant treatment, and sperm cryopreservation but resulted with low rates of satisfaction. Procarbazine is a well known agent causing sterility even in the first doses of chemotherapy. Antioxidants such as N acetylcysteine and ascorbate have been used for protective purposes and were very successful. Melatonin (M) is another powerful antioxidant and we aimed to use M for the protection of P induced testicular toxicity in this study. Procarbazine was given peroral by gavage once a week at a dose of 62.5 mg/kg/week for 4 weeks (total dose: 250 mg/kg) (P group) and in procarbazine + melatonin (PM) group, 10 mg/kg melatonin was intraperitoneally administered daily for five days a week for 4 weeks (total 20 days). The experiment ended at day 90. In the P and PM groups the testicle width, length, and weight, sperm A and sperm AB properties (Sperm A: sperms straight line progressive, Sperm B: sperms straight slow progressive, Sperm AB: Sperm A + Sperm B), spermatogonia, Sertoli cells, seminiferous tubule, and germinative layer thickness were lowered as compared with the control group. However, there were no significant differences between the P and PM groups in regard to these parameters. Melatonin preserved Sertoli cell and spermatogonia function. The testosterone and follicle-stimulating hormone (FSH) levels were also preserved. Melatonin significantly decreased malondialdehyde (MDA) levels and preserved the antioxidant enzyme levels such as glutathione peroxidase (GPx) and nitrite nitrate (NO2-/NO3-). Melatonin may protect testicular functions in P treated patients and is open to consideration during chemotherapy since it appears to be without any side effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c6b0fa810166da67c692501e3b03434 https://doi.org/10.3109/19396368.2014.930212 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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