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White rot fungi are the only organisms capable of mineralizing lignin. This is possible thanks to the unspecificity of the ligninolytic enzymes which are able to oxidize a wide range of substrates. These enzymes have several applications like biorremediaton of environments contaminated with textile dyes. The aim of this invatigation was to determine laccases and total peroxidases actitivies and the capacity of the extract to decolorize textile dyes (Acid Red 1, AR1; Remazol Brilliant Blue R, RBBR; Acid Blue 129, AB129; Reactive Blue 4, RB4 e Reactive Black 5, RB5) of two white rot fungi isolated form rotten logs on the campus of the Federal University of the Amazonas State (UFAM). The isolates were cultivated in broth PD (potato dextrose) and after 18 days cultivation the micelium was separated from que extract in order to determine micelial dry weight and enzyme activity. Posteriorly the isolates were culti- vated in the same medium with each one of the dyes added separately (50mg/L). After 18 days cultivation isolates 15 and 18 presented laccase activity (UI/L) of 70,2 and 73,2 respectively, and total peroxidase activity of 40,4 and 87,9 respectively. Both isolates were able to decolorate all dyes and the highest decoloration was observed on RB5 (isolate 15, 93,2% and isolate 18, 95,5%) while the lowest was observered on AR1 (isolate 15, 20,0% and isolate 18, 21,3%). The fungi used on this investigation showed potential to be used in biorremediation systems. |
