Solvolytic depolymerization of chondroitin and dermatan sulfates
| Autor: | Kenji Sato, Shinobu Sakai, Saori Hosoyama, Robert J. Linhardt, Toshihiko Toida, Noriko Sakamoto |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Molecular Structure Depolymerization Organic Chemistry Disaccharide Dermatan Sulfate Oligosaccharides General Medicine Oligosaccharide Biochemistry Dermatan sulfate Analytical Chemistry carbohydrates (lipids) Glycosaminoglycan chemistry.chemical_compound Residue (chemistry) Structure-Activity Relationship chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Chondroitin Organic chemistry Chondroitin sulfate |
| Zdroj: | Carbohydrate research. 344(7) |
| ISSN: | 1873-426X |
| Popis: | It is essential to establish a library of glycosaminoglycan oligosaccharides from the chondroitin and dermatan sulfates to investigate their biological functions and structure-activity relationships (SARs). There are several approaches to obtain oligosaccharides using chemical and enzymatic degradation procedures; however, purification of each resulting oligosaccharide is complicated because of the diversity of sulfonation patterns present in these oligosaccharides. We have developed a new method for the solvolytic degradation for chondroitin and dermatan sulfates to obtain an oligosaccharide mixture that can be easily purified into chondro/dermato oligosaccharides for characterization by both (1)H NMR and MALDI-TOFMS. These oligosaccharides have a methyl-esterified uronate residue and a methyl 2-acetamido-2-deoxy-D-galactofuranoside at the nonreducing and reducing ends, respectively. All other internal repeating disaccharide units were desulfonated, but maintained their core carbohydrate structures. |
| Databáze: | OpenAIRE |
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