Unraveling human adult hippocampal neurogenesis
| Autor: | Julia Terreros-Roncal, Elena P. Moreno-Jiménez, Jesús Avila, Miguel Flor-García, María Llorens-Martín, Alberto Rábano |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Alzheimer's Association, Association for Frontotemporal Degeneration (US), Comunidad de Madrid, Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas (España), Fundación Ramón Areces, Banco Santander |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
education.field_of_study Future studies Neurogenesis Population Hippocampus Biology Hippocampal formation Mammalian brain General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Immunohistochemistry education Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Adult neurogenesis occurs in a few selected regions of the mammalian brain. One such region is the hippocampus, the so-called gateway to memory, where adult hippocampal neurogenesis (AHN) occurs. Here, we provide a comprehensive description of the methods used in our laboratory to unambiguously detect a population of immature neurons in the human hippocampus until the 10th decade of life. The criteria used to refine and develop the current protocol include obtaining post-mortem human samples of remarkable quality and under tightly controlled conditions for immunohistochemistry (IHC) studies, optimizing tissue processing and histological procedures, establishing criteria to reliably validate antibody signal and performing unbiased stereological cell counts. Moreover, we provide a detailed description of the parameters that, in our view, should be reported in human AHN studies. The opposing results obtained by introducing slight variations in the methodological conditions should be considered by future studies that seek to increase our knowledge of this fascinating process. By applying simple and inexpensive tissue pre-treatments, this protocol, which can be completed in 7 days, might be applicable to a variety of IHC studies performed on other tissues of human (or animal) origin. the Spanish Ministry of Economy and Competitiveness (SAF-2017-82185-R and RYC-2015-171899 (M.L.-M.); SAF-2014-53040-P (J.A.)); The Alzheimer´s Association (2015-NIRG-340709 and AARG-17-528125 (M.L.-M.)); The Association for Frontotemporal Degeneration (2016 Basic Science Pilot Grant Award (M.L.-M.)); the Comunidad de Madrid (PEJD-2017-PRE/BMD-3439 (M.L.-M.)); and the Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED, Spain) (J.A.). Institutional grants from the Fundación Ramón Areces and Banco de Santander |
| Databáze: | OpenAIRE |
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