The effects of dimethyl sulfoxide on the osmotic properties of a rat megakaryocytopoietic cell line
| Autor: | Andrew Connolly, H.G. Hempling |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Cell Membrane Permeability
Cell fusion Membrane permeability Chemistry Dimethyl sulfoxide Cellular differentiation Water Megacaryocyte General Medicine In Vitro Techniques General Biochemistry Genetics and Molecular Biology Cell Line Rats Cell biology chemistry.chemical_compound Osmotic Pressure Cell culture Animals Osmotic pressure Dimethyl Sulfoxide General Agricultural and Biological Sciences Megakaryocytes Intracellular |
| Zdroj: | Cryobiology. 22:351-358 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(85)90182-8 |
| Popis: | Dimethyl sulfoxide (DMSO) at 0.6 M alters the osmotic state of intracellular water in a line of rat megakaryocytopoietic cells. The response is a function of temperature. Maximal structuring of water occurs at 24.7 degrees C, while disorganization occurs at 30 and 37 degrees C. Little effect occurs at 3 and at 18 degrees C. Parallel measurements of membrane permeability to water indicate that DMSO inhibits the osmotic exit of water at all temperatures. Maximal inhibition occurs at 24.7 degrees C, the same temperature at which maximal organization of water occurs. These findings emphasize the possible role which water may play in explaining the diverse functions of DMSO as cryogenic substance, promoter of cell fusion, and stimulator of cell differentiation. |
| Databáze: | OpenAIRE |
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