The order of immunoglobulin light chain κ and λ usage in primary and secondary lymphoid tissues of germ-free and conventional piglets

Autor: Katerina Stepanova, Jana Sinkorova, Dagmar Srutkova, Marek Sinkora, Simon Sinkora, Igor Splichal, Alla Splichalova, John E. Butler
Rok vydání: 2022
Předmět:
Zdroj: Developmental & Comparative Immunology. 131:104392
ISSN: 0145-305X
DOI: 10.1016/j.dci.2022.104392
Popis: In pigs (Sus scrofa), the initial immunoglobulin rearrangement of the κ light chain is replaced by λ before the heavy chains rearrange, and the light chains may rearrange even later. This study investigates whether these developmental differences are reflected in the usage of IGK and IGL genes. We found large differences between peripheral B cells and those developing in the bone marrow, and between B cells in germ-free piglets and conventional pigs. During early B cell development in the bone marrow, more 3' V and 5' J gene segments for both light chains are used. However, in the peripheral naive repertoire, more 5' IGLV and 3' IGLJ genes are used. A similar shift toward the use of more 5' IGKV and 3' IGKJ genes is observed later after antigen exposure in conventional pigs. The expression profile showed that most λ
Databáze: OpenAIRE
Externí odkaz: