Quick and cheap MIRU-VNTR typing of Mycobacterium tuberculosis species complex using duplex PCR
| Autor: | Jessica L. de Beer, Rubina Tabassum Siddiqui, Christophe Sola, Memona Yasmin, Stéphanie Le Moullec, Guislaine Refrégier |
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| Přispěvatelé: | Health Biotechnology Division [NIBGE], NIBGE, National Institute for Biotechnology and Genetic Engineering ( NIBGE ) -National Institute for Biotechnology and Genetic Engineering ( NIBGE ), Pakistan Institute of Engineering and Applied Sciences ( PIEAS ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 ), National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment [Bilthoven] ( RIVM ), National Institute for Biotechnology and Genetic Engineering (NIBGE)-National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan Institute of Engineering and Applied Sciences (PIEAS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), National Institute for Public Health and the Environment [Bilthoven] (RIVM) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Genotyping Genotyping Techniques [SDV]Life Sciences [q-bio] 030106 microbiology Immunology Locus (genetics) Minisatellite Repeats Multiple Loci VNTR Analysis Microbiology Multiplexing Mycobacterium tuberculosis 03 medical and health sciences Genotype Humans Tuberculosis Genetics Electrophoresis Agar Gel biology [ SDV ] Life Sciences [q-bio] MLVA Amplicon biology.organism_classification 3. Good health Bacterial Typing Techniques Infectious Diseases Minisatellite Agarose gel electrophoresis Multiplex Polymerase Chain Reaction |
| Zdroj: | Tuberculosis (Edinburgh, Scotland) Tuberculosis (Edinburgh, Scotland), 2016, 101, pp.160-163. 〈10.1016/j.tube.2016.10.003〉 Tuberculosis Tuberculosis, Elsevier, 2016, 101, pp.160-163. ⟨10.1016/j.tube.2016.10.003⟩ Tuberculosis, 2016, 101, pp.160-163. ⟨10.1016/j.tube.2016.10.003⟩ |
| ISSN: | 1472-9792 |
| DOI: | 10.1016/j.tube.2016.10.003〉 |
| Popis: | International audience; While minisatellites are usually typed using capillary sequencers or qiaplex systems in developed countries, many low-resource regions cannot afford it. We propose an optimized agarose gel electrophoresis method to genotype Mycobacterium tuberculosis species complex minisatellites in their standardized format (24 MIRU-VNTR). It is based on duplex PCRs combining VNTR loci harboring distinct amplicon sizes whatever the repetition number of each locus. This method performs well both on DNA extracts of good quality and on thermolysates while reducing workload and reagents costs. |
| Databáze: | OpenAIRE |
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