Anticancer activity ofCocculus hirsutusagainst Dalton’s lymphoma ascites (DLA) cells in mice

Autor:	Molly Mathew, Dhanabal S. Palaniswamy, B. Samuel Thavamani
Rok vydání:	2014
Předmět:	Male Lymphoma Cell Survival Ethyl acetate Pharmaceutical Science Cell Count Antioxidants Superoxide dismutase Cocculus hirsutus Mice chemistry.chemical_compound Alkaloids Phenols Cell Line Tumor Drug Discovery Animals Humans Petroleum ether MTT assay Menispermaceae Flavonoids Pharmacology Cell Death biology Traditional medicine Plant Extracts Superoxide Dismutase Alkaloid Cocculus General Medicine Catalase biology.organism_classification Antineoplastic Agents Phytogenic Complementary and alternative medicine chemistry Biochemistry Phytochemical Solvents biology.protein Molecular Medicine Female Lipid Peroxidation Drug Screening Assays Antitumor
Zdroj:	Pharmaceutical Biology. 52:867-872
ISSN:	1744-5116 1388-0209
DOI:	10.3109/13880209.2013.871642
Popis:	Cocculus hirsutus (L.) Diels (Menispermaceae) is used in Indian folk system of alternative medicine for rheumatism, eczema, diabetics, inflammation, and neuralgia.To evaluate antitumor activities of C. hirsutus in vitro and in vivo.C. hirsutus was successively extracted using hexane, petroleum ether, chloroform, ethyl acetate, methanol, and water. In vitro cytotoxicity was assessed by the MTT assay. Phytochemical analyses were conducted with methanol extract of C. hirsutus (MECH) and in vivo antitumor activity was carried out with MECH using Dalton's lymphoma ascites (DLA) mouse model. Antioxidant properties were assessed by estimating superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation.Phytochemical studies indicated a high content of total alkaloid (165.6 mg/100 g), total phenolic (43.5 GAE mg/g), and total flavanoid (4.97 RE mg/g) in MECH. Anti-proliferative activity against the breast cancer cell line MCF-7 showed IC50 values of 221.5 ± 16.68, 255 ± 17.88, 213 ± 8.4, 147 ± 7.9, and 229 ± 8.02 µg/ml with hexane, petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts, respectively. A significant (p 0.01) decrease in packed cell volume, viable cell count, and increased lifespan (58 and 77%) was observed. Hematological and serum biochemical profiles were restored to normal levels in MECH-treated mice. MECH-treated group significantly (p 0.001) decreased SOD, lipid peroxidation, and CAT towards normal.C. hirsutus exhibited significant in vitro and in vivo antitumor activities that are reasonably attributed to endogenous antioxidant mechanisms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c5faecfe655bfe0029b815294daf921 https://doi.org/10.3109/13880209.2013.871642 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.