Articular cartilage restoration in load-bearing osteochondral defects by implantation of autologous chondrocyte-fibrin constructs: an experimental study in sheep
| Autor: | O C Samsudin, Bin Saim Aminuddin, B.H.I. Ruszymah, S Munirah, Hui Cheng Chen, S. H. Sharifah Salmah |
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| Rok vydání: | 2007 |
| Předmět: |
Cartilage
Articular Pathology medicine.medical_specialty education Articular cartilage Biocompatible Materials Transplantation Autologous Fibrin Chondrocyte Load bearing Chondrocytes Biopsy Materials Testing Medicine Animals Regeneration Orthopedics and Sports Medicine Cartilage repair Cells Cultured Sheep biology medicine.diagnostic_test Tissue Scaffolds business.industry Cartilage Regeneration (biology) Anatomy Stifle Disease Models Animal medicine.anatomical_structure biology.protein Surgery business |
| Zdroj: | The Journal of bone and joint surgery. British volume. 89(8) |
| ISSN: | 0301-620X |
| Popis: | Ovine articular chondrocytes were isolated from cartilage biopsy and culture expanded in vitro. Approximately 30 million cells per ml of cultured chondrocytes were incorporated with autologous plasma-derived fibrin to form a three-dimensional construct. Full-thickness punch hole defects were created in the lateral and medial femoral condyles. The defects were implanted with either an autologous ‘chondrocyte-fibrin’ construct (ACFC), autologous chondrocytes (ACI) or fibrin blanks (AF) as controls. Animals were killed after 12 weeks. The gross appearance of the treated defects was inspected and photographed. The repaired tissues were studied histologically and by scanning electron microscopy analysis. All defects were assessed using the International Cartilage Repair Society (ICRS) classification. Those treated with ACFC, ACI and AF exhibited median scores which correspond to a nearly-normal appearance. On the basis of the modified O’Driscoll histological scoring scale, ACFC implantation significantly enhanced cartilage repair compared to ACI and AF. Using scanning electron microscopy, ACFC and ACI showed characteristic organisation of chondrocytes and matrices, which were relatively similar to the surrounding adjacent cartilage. Implantation of ACFC resulted in superior hyaline-like cartilage regeneration when compared with ACI. If this result is applicable to humans, a better outcome would be obtained than by using conventional ACI. |
| Databáze: | OpenAIRE |
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