Persistent Site-Specific Remodeling of a Nucleosome Array by Transient Action of the SWI/SNF Complex
| Autor: | Tom Owen-Hughes, Jacques Côté, Craig L. Peterson, Rhea T. Utley, Jerry L. Workman |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Saccharomyces cerevisiae Proteins
cells Molecular Sequence Data genetic processes Saccharomyces cerevisiae macromolecular substances Fungal Proteins Histones Adenosine Triphosphate Transcription (biology) Deoxyribonuclease I Nucleosome Chromatin structure remodeling (RSC) complex DNA Fungal Transcription factor Adenosine Triphosphatases Binding Sites Multidisciplinary Base Sequence biology SWI/SNF complex Nuclear Proteins SWI/SNF Nucleosomes Chromatin Cell biology DNA-Binding Proteins enzymes and coenzymes (carbohydrates) Histone Biochemistry biology.protein biological phenomena cell phenomena and immunity Transcription Factors |
| Zdroj: | Science. 273:513-516 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.273.5274.513 |
| Popis: | The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling of a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes. |
| Databáze: | OpenAIRE |
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