A promising radiolabeled drug delivery system for methotrexate: synthesis and in vitro evaluation of 99mTc labeled drug loaded uniform mesoporous silica nanoparticles
| Autor: | Emin Ilker Medine, Serap Teksöz, Selin İrdel Hamurişçi, Çiğdem İçhedef, Burcu Aydın |
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| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
Drug animal structures Carrier system Mesoporous silica nanoparticles Health Toxicology and Mutagenesis media_common.quotation_subject Drug delivery system Pharmacology Analytical Chemistry Biodistribution In vitro Size medicine Cytotoxic T cell Radiology Nuclear Medicine and imaging skin and connective tissue diseases Spectroscopy media_common A549 cell Chemistry Public Health Environmental and Occupational Health Mesoporous silica Pollution Methotrexate Colloidal Suspensions Nuclear Energy and Engineering Drug delivery sense organs Radiolabeling medicine.drug |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 330:1113-1125 |
| ISSN: | 1588-2780 0236-5731 |
| Popis: | In present study, we describe a promising radiolabeled drug delivery system for Methotrexate (MTX), an anticancer drug used in the treatment of breast cancer. Uniform and re-dispersible MSN were synthesized with a particle size of as 42.55 +/- 1.45 nm. Then, MTX was loaded into the surface modified MSN with DTPA over 95% loading capacity. Subsequently, MTX loaded MSN carrier system was radiolabeled with Tc-99 m (Tc-99m-MTX-MSN) with 92.20 +/- 0.8% radiolabeling yield. Furthermore, in vitro evaluation on estrogen positive (ER +) MCF7 and estrogen negative (ER-) A549 cells lines were performed for determining apoptotic and cytotoxic effects of MTX-MSN, and incorporation behavior of Tc-99m-MTX-MSN. Drug loaded MSN particles were exhibit higher uptake in MCF7 cells than A549 cells. Ege University Scientific Research Projects Coordination Unit [2017NBE006] The authors gratefully acknowledge the support by Ege University Scientific Research Projects Coordination Unit (Funding Number: 2017NBE006). |
| Databáze: | OpenAIRE |
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