Punching a Graft for Descemet Membrane Endothelial Keratoplasty Onto a Contact Lens Reduces Endothelial Cell Loss at the Graft's Margin
| Autor: | Eitan Livny, Asaf Achiron, Irit Bahar, Yoav Nahum, Yael Nisgav, Avital Adler |
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| Rok vydání: | 2020 |
| Předmět: |
Graft Rejection
Male medicine.medical_specialty Descemet membrane Contact Lenses Chemistry Endothelium Corneal Fuchs' Endothelial Dystrophy Visual Acuity Endothelial stem cell Transplantation Contact lens Ophthalmology surgical procedures operative Stroma medicine Humans Female Punching Descemet Stripping Endothelial Keratoplasty Aged Retrospective Studies |
| Zdroj: | Cornea. 39:1027-1030 |
| ISSN: | 0277-3740 |
| Popis: | Purpose To evaluate whether punching Descemet membrane endothelial keratoplasty (DMEK) corneal grafts onto a contact lens scaffold reduces endothelial cell loss at the graft margin in comparison to punching the graft directly onto the donor stroma. Methods DMEK grafts were prepared using 2 different methods after peeling the graft from the stroma: punching onto a contact lens and punching onto the donor stroma. The grafts were then evaluated for the width of Descemet membrane devoid of endothelial cells in the peripheral ring, measured at 4 points at the graft margin. Results Our study included 6 grafts, harvested from 3 donors aged 66.3 ± 5.1 years. Grafts prepared on a contact lens scaffolding had more of their Descemet membrane margin populated by endothelial cells than did grafts that were punched directly onto the donor stroma (total denuded area: 0.06 ± 0.08 mm vs. 1.17 ± 0.02 mm, P = 0.018; maximal width of denuded area: 59.6 ± 28.4 μm vs. 100.2 ± 59.7 μm, P = 0.07). Donor grafts on contact lens had approximately 2.5% more endothelial cells available for transplantation (2425 cells/mm vs. 2367 cells/mm). Graft preparation time did not significantly differ between the methods (6.4 ± 0.49 vs. 9.8 ± 3.7 minutes, P = 0.46). Conclusions Punching DMEK grafts onto a contact lens reduces endothelial loss at the grafts' margins and may prolong their survival. |
| Databáze: | OpenAIRE |
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