Reversed-phase ion-pair chromatography coupled to electrospray ionisation mass spectrometry by on-line removal of the counter-ions
| Autor: | Niklas Tyrefors, Jenny Samskog, Bengt Långström, Benita H. Forngren, Karin E. Markides, Sven Gustavsson |
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| Rok vydání: | 1999 |
| Předmět: |
Ions
Electrospray Chromatography Protein mass spectrometry Chemistry Electrospray ionization Organic Chemistry Extractive electrospray ionization Analytical chemistry General Medicine Mass spectrometry Biochemistry High-performance liquid chromatography Mass Spectrometry Sample preparation in mass spectrometry Analytical Chemistry Liquid chromatography–mass spectrometry Radiometry Anion Exchange Resins Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 854:155-162 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(99)00526-9 |
| Popis: | A strong anion-exchanger was used as a trapping column to perform on-line coupling of ion-pair chromatography with electrospray ionisation mass spectrometry. Ion-pairing reagents were used to retain polar analytes of low molecular mass away from the solvent front in reversed-phase LC. The trapping column enabled removal of the non-volatile counter-ions from the mobile phase prior to detection, so that the electrospray process could be performed with favourable ionisation conditions and without contamination of the interface. The efficiency of the trapping process was studied for 1-octanesulfonic acid and sodium dodecyl sulfate as ion-pairing reagents. Using this on-line trapping method, biopterin and guanidine could be retained with a k'>2 and detected by electrospray mass spectrometry with a stable signal. |
| Databáze: | OpenAIRE |
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