Oncolytic Viral Therapy for Human Prostate Cancer by Conditionally Replicating Herpes Simplex Virus 1 Vector G207
| Autor: | Masafumi Oyama, Takashi Ohigashi, Masaru Murai, Keiichi Uyemura, Michio Hoshi, Takahito Yazaki |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Oncolytic virus Genetic Vectors Mice Nude Herpesvirus 1 Human Herpes simplex virus Biology Transfection Virus Replication urologic and male genital diseases Article Mice Prostate cancer Gene therapy Multiplicity of infection DU145 Prostate Tumor Cells Cultured medicine Animals Humans Prostate neoplasms Replication‐competent virus Prostatic Neoplasms Cancer Genetic Therapy medicine.disease medicine.anatomical_structure Oncology Adenocarcinoma Prostate neoplasm Cell Division |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| DOI: | 10.1111/j.1349-7006.2000.tb00923.x |
| Popis: | Over the last few years, a conditionally replicating herpes simplex virus 1 (HSV-1) vector, G207 has been used for the treatment of several malignant tumors. In this article we evaluate the anti-tumoral effect of G207 against prostate cancer in vitro and in vivo. The susceptibility of the human prostate cancer cell lines, DU145 and PC3 to G207 at a multiplicity of infection (MOI) of 0.1 was examined. In addition, the growth characteristics of G207 were assessed. Athymic mice with s.c. tumors were inoculated in vivo intraneoplastically with 1 x 10(7) plaque-forming units (PFU) of G207. For the pathological analyses, s.c. tumors were stained with X-gal. DU145 and PC3 were efficiently destroyed by G207 within 7 days. The viral yields of G207 increased time-dependently. In vivo, the intraneoplastic inoculation of G207 induced a significant inhibition of the tumor growth. The mean tumor growth ratio was significantly inhibited in the G207-treated tumors (DU145, P < 0.0001; PC3, P < 0.001 versus controls). In a pathological study, many lacZ-positive cells were diffusely present in the G207-treated tumors. G207 showed a significant antitumoral effect against human prostate cancer cell lines, and thus may be considered a useful agent for the treatment of prostate cancer. |
| Databáze: | OpenAIRE |
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