Programmed cell death-associated gene transcripts in bovine embryos exposed to bovine Herpesvirus type 5
| Autor: | Camila Silva-Frade, Tereza C. Cardoso, Lucas Hidenori Okamura, Roberto Gameiro, Eduardo Furtado Flores |
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| Rok vydání: | 2014 |
| Předmět: |
Programmed cell death
Cattle Diseases Apoptosis Mitochondrion Biology Real-Time Polymerase Chain Reaction Transcription (biology) medicine Animals Molecular Biology Gene Herpesvirus 5 Bovine Gene Expression Regulation Developmental Embryo Herpesviridae Infections Cell Biology Embryo Mammalian Oocyte Molecular biology In vitro Genes Mitochondrial medicine.anatomical_structure Oocytes Myeloid Cell Leukemia Sequence 1 Protein Cattle |
| Zdroj: | Molecular and Cellular Probes. 28:113-117 |
| ISSN: | 0890-8508 |
| DOI: | 10.1016/j.mcp.2013.10.005 |
| Popis: | In vitro-produced bovine embryos become infected after exposure to bovine Herpesvirus type 5 (BoHV-5), yet no changes in developmental rates, mitochondrial activity and inhibition of apoptosis are detected in comparison to unexposed embryos. Thus, the aim of the present study was to assess the transcription of mitochondria-mediated apoptosis genes using TaqMan real-time polymerase chain reaction. Transcripts of mcl-1, caspase-2, -3, Apaf-1 and Bax genes were measured after exposure to BoHV-5 in vitro. Mitochondrial dehydrogenase activity was evaluated by MTT test and compared between groups of exposed and unexposed embryos, at day 7 of development. The rate of oocyte maturation was assessed by the extrusion of the first polar body. In summary, BoHV-5 exposed embryos retained their viability, mitochondrial dehydrogenase activity and displayed up-regulation of transcription of survival mcl-1 gene and down-regulation of Bax transcription in relation to mitochondria-mediated pathway which might improve embryo viability. These findings demonstrate that BoHV-5 exposed embryos maintain their viability and mitochondrial dehydrogenase activity with no compromise of embryos produced in vitro. |
| Databáze: | OpenAIRE |
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