Antioxidant properties of albumin: effect on oxidative metabolism of human neutrophil granulocytes
| Autor: | Ferdinand Kouoh, Bernard Gressier, Micheline Cazin, Thierry Dine, Claude Brunet, Jean Claude Cazin, Michel Luyckx |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Antioxidant
Neutrophils medicine.medical_treatment Serum albumin Pharmaceutical Science Granulocyte medicine.disease_cause Antioxidants Superoxides Drug Discovery medicine Animals Humans Bovine serum albumin chemistry.chemical_classification Reactive oxygen species biology Albumin Serum Albumin Bovine Biological activity Hydrogen Peroxide Hypochlorous Acid medicine.anatomical_structure chemistry Biochemistry biology.protein Cattle Oxidative stress |
| Zdroj: | Il Farmaco. 54:695-699 |
| ISSN: | 0014-827X |
| Popis: | The present study aims at investigating the effect of bovine serum albumin (BSA) on the trial of oxidative-stress. The antioxidant effects of BSA were determined by human neutrophil granulocytes oxygen free radicals and their by-products (O − 2 , H 2 O 2 , HOCl) productions. BSA interacts with those reactive oxygen species (ROS) in a dose-dependent manner. The 50% inhibitory concentration (IC 50 ) of BSA estimated, after phorbol-12-myristate-13-acetate (PMA) stimulation were: 33.5 mg/ml for O − 2 , 6.5 mg/ml for H 2 O 2 , and 6.85 mg/ml for HOCl. When neutrophils were washed after pre-incubation with BSA, there was no significant decrease of ROS after stimulation of PMA (maximal: 15±1.2%). In the free cell experiments, IC 50 for H 2 O 2 and HOCl were 7.86 mg/ml and 0.67 mg/ml, respectively. The mechanism at which BSA acts may result from a simple chemical interaction with ROS rather than an intracellular mechanism by intervention in PMA oxidative metabolism. These antioxidant activities confer to BSA properties, which might be used to prevent damage inflicted by these ROS during inflammatory disorders. |
| Databáze: | OpenAIRE |
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