Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae
| Autor: | E L Ivanov, F Fabre, James E. Haber, Neal Sugawara, Charles I. White |
|---|---|
| Přispěvatelé: | Rosenstiel Basic Medical Sciences Research Center [Waltham], Brandeis University, Section de Biologie [Institut Curie] (Centre Universitaire Orsay), Institut Curie [Paris]-Centre Universitaire d’Orsay, This work was supported by NIH grant GM20056 (J.E.H.) and Institut Curie (F.F.)., White, Charles |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
[SDV]Life Sciences [q-bio]
RAD52 Mutant Restriction Mapping [SDV.GEN] Life Sciences [q-bio]/Genetics medicine.disease_cause Polymerase Chain Reaction 0302 clinical medicine Strand invasion DNA Fungal Deoxyribonucleases Type II Site-Specific Genetics 0303 health sciences Mutation biology [SDV] Life Sciences [q-bio] DNA-Binding Proteins Enzyme Induction biological phenomena cell phenomena and immunity Research Article Saccharomyces cerevisiae Proteins Genotype Saccharomyces cerevisiae Genes Fungal Molecular Sequence Data Fungal Proteins 03 medical and health sciences Open Reading Frames medicine Point Mutation Amino Acid Sequence Molecular Biology Crosses Genetic 030304 developmental biology DNA Primers [SDV.GEN]Life Sciences [q-bio]/Genetics Base Sequence Cell Biology biology.organism_classification Genes Mating Type Fungal Methyl Methanesulfonate enzymes and coenzymes (carbohydrates) Kinetics Mating of yeast Mutagenesis Rad50 Homologous recombination 030217 neurology & neurosurgery Gene Deletion |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 1994, 14 (5), pp.3414-25 |
| ISSN: | 0270-7306 1098-5549 |
| Popis: | International audience; In Saccharomyces cerevisiae, a large number of genes in the RAD52 epistasis group has been implicated in the repair of chromosomal double-strand breaks and in both mitotic and meiotic homologous recombination. While most of these genes are essential for yeast mating-type (MAT) gene switching, neither RAD50 nor XRS2 is required to complete this specialized mitotic gene conversion process. Using a galactose-inducible HO endonuclease gene to initiate MAT switching, we have examined the effect of null mutations of RAD50 and of XRS2 on intermediate steps of this recombination event. Both rad50 and xrs2 mutants exhibit a marked delay in the completion of switching. Both mutations reduce the extent of 5'-to-3' degradation from the end of the HO-created double-strand break. The steps of initial strand invasion and new DNA synthesis are delayed by approximately 30 min in mutant cells. However, later events are still further delayed, suggesting that XRS2 and RAD50 affect more than one step in the process. In the rad50 xrs2 double mutant, the completion of MAT switching is delayed more than in either single mutant, without reducing the overall efficiency of the process. The XRS2 gene encodes an 854-amino-acid protein with no obvious similarity to the Rad50 protein or to any other protein in the database. Overexpression of RAD50 does not complement the defects in xrs2 or vice versa. |
| Databáze: | OpenAIRE |
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